Comparison of Two Methods of LPS-Induced Two-Hit Acute Lung Injury Models and Analysis of Clinical Syndrome Characteristics in Chinese and Western Medicine
- VernacularTitle:两种LPS"二次打击"方式建立急性肺损伤的模型比较及中西医临床病证特点分析
- Author:
Guofeng CHEN
1
;
Junqing LIANG
;
Xu HE
;
Xiaojia HUANG
;
Ye LIN
;
Jing SUN
;
Yan LI
Author Information
- Publication Type:Journal Article
- Keywords: Lipopolysaccharide; Two-hit; Acute lung injury model; Chinese and Western medicine; Clinical syndrome characteristics
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(8):2340-2350
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the efficacy of two lipopolysaccharide(LPS)two-hit methods in establishing an acute lung injury(ALI)mouse model and analyze the characteristics of this model within the context of both Western and traditional Chinese medicine(TCM).Methods Healthy male C57BL/6 mice were randomly divided into six groups:PBS instillation control group,PBS nebulization control group,instillation two-hit group,nebulization two-hit group I,nebulization two-hit group Ⅱ,and nebulization two-hit group Ⅲ.Using LPS as a stimulant,a"two-hit"approach was employed to establish an ALI mouse model.Body temperature,weight,pulse oximetric oxygen saturation(SpO2),lung tissue wet-to-dry weight ratio(W/D),levels of tumor necrosis factor(TNF-α),interleukin-1β(IL-1β),and myeloperoxidase(MPO)activity in lung tissue,as well as total protein concentration and white blood cell count in bronchoalveolar lavage fluid were measured.Through evaluating the consistency between TCM and Western medical syndromes,the classification,characteristics,modeling methods,advantages,and disadvantages of the two-hit ALI animal model were summarized and analyzed based on the clinical diagnostic criteria and syndrome characteristics of ALI in both TCM and Western medicine.Results Compared with the control group,the body temperature,weight,and SpO2 of model group decreased,W/D increased,levels of TNF-α,IL-1β,and MPO activity in lung tissue increased.The alveolar walls thickened with a large exudate of red blood cells.The total protein concentration and white blood cell count in bronchoalveolar lavage fluid significantly increased.The existing two-hit ALI animal model showed a high degree of consistency with Western medical diagnostic main symptoms.Conclusion Both methods of two-hit can prepare mouse ALI models,among which the nebulization two-hit Group Ⅲ showed more pronounced effects in simulating the pathological changes and clinical symptoms of ALI.
