Development of the animal model of trauma brain injury coupled with acute lung injury induced by seawater drowning
10.3760/cma.j.issn.1009-6906.2019.04.002
- VernacularTitle:大鼠创伤性脑损伤合并海水淹溺性急性肺损伤模型的建立
- Author:
Wangwang ZHONG
1
;
Liangfeng WEI
;
Weiqiang CHEN
;
Tianfei LI
;
Hao ZHANG
;
Jun TIAN
;
Shousen WANG
Author Information
1. 350025福州,解放军联勤保障部队第九○○医院神经外科
- Publication Type:Journal Article
- Keywords:
Animal model;
Rat;
Traumatic brain injury;
Seawater drowning;
Acute lung injury
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(4):267-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an animal model of traumatic brain injury coupled with acute lung injury induced by seawater drowning.Methods A total of 80 male Sprague-Dawley rats were randomly divided into 4 groups:the sham group,the traumatic brain injury (TBI) group,the seawater drowning-induced acute lung injury (SWD-ALI) group and the traumatic brain injury coupled with seawater drowning-induced acute lung injury (TBI + SWD-ALI) group,each consisting of 20 animals.The TBI model group was established with the impact power of 450 g × 1.5 m by the " Marmarou method".The SWD-ALI group was established by pumping the artificial seawater into the rat trachea at a rate of 3 ml/kg.The TBI + SWD-ALI group was established on the basis of TBI.The data of arterial blood gases were detected 10,30,60,120 and 240 minutes after trauma injury.Wet/dry weight of the lung was detected 240min after injury,and pulmonary pathological changes were also observed after HE staining.Pathological changes in the brain tissue after HE staining and the expression levels of/3-APP and NF-L after immunohistochemical (IHC) staining were observed 24 hours after injury.Results The levels of PaO2 after injury during observation period generally displayed an increasing trend in the TBI,the SWD-ALI and the TBI + SWD-ALI groups.The levels of PaO2 10 minutes after injury were the lowest,which were respectively (85.12 ± 2.03) mmHg,(37.75 ± 1.17) mmHg and (33.38 ± 1.92) mmHg,and were obviously lower than that of the sham group (95.25 ± 2.05) mmHg,with statistical significance (P＜0.05).The levels of PaO2 at the time point of 240 minutes after injury reached peak,which were respectively (96.63 ± 1.60) mmHg,(86.13 ± 2.03) mmHg and (77.25 ± 3.33) mmHg,and the levels of PaO2 for the SWD-ALI and TBI + SWD-ALI groups were significantly lower than that of the sham group [(97.50 ± 1.20) mmHg],also with statistical significance (P ＜ 0.05).The levels of PaO2 in the TBI + SWD-ALI group was considerably lower than that of the SWD-ALI group (P ＜ 0.05).The ratios of wet/ dry weight of the lung tissue at the time point of 240 minutes after injury for the SWD-ALI group and the TBI + SWD-ALI group were respectively (7.30 ± 0.16) and (7.34 ± 0.21),which were all obviously higher than those of the sham group (4.81 ±0.18) and the TBI group (4.91 ±0.18),also with statistical significance (P ＜ 0.05).However,no statistical significance could be seen,when comparisons were made between the SWD-ALI and TBI + SWD-ALI groups (P ＞ 0.05).HE staining indicated that such pulmonary pathological lesions as wider alveolar septa,alveolar space fusion,inflammatory cell infiltration in various degrees could be observed in the SWD-ALI and TBI + SWD-ALI groups.In addition,brain tissue HE staining revealed that neuronal cell degeneration in the cortex of the brain tissue could be detected in the TBI,SWD-ALI and TBI + SWD-ALI groups,and immunohistochemical staining indicated that there were positive expressions of β-APP and NF-L in the TBI and TBI + SWD-ALI groups.Conclusion The impact with the power of 450 g × 1.5 m by the "the Marmarou method" followed by pumping of seawater (3 ml/kg) into trachea could establish a stable and reproductive rat model of traumatic brain injury coupled with acute lung injury induced by seawater drowning.
