MicroRNA analysis of the regulatory effects of Gualou Xiebai Banxia Decoction on the Epsin1-fibroblast growth factor receptor 1 pathway in atherosclerotic mouse
10.3969/j.issn.1671-7856.2024.11.004
- VernacularTitle:基于miRNA测序探究瓜蒌薤白半夏汤对动脉粥样硬化小鼠Epsin1-FGFR1通路调节机制研究
- Author:
Zhaobo WANG
1
;
Yi PAN
1
;
Qian LIN
1
Author Information
1. 北京中医药大学东直门医院,北京 100700
- Publication Type:Journal Article
- Keywords:
coronary heart disease;
atherosclerosis;
miRNA;
Gualou Xiebai Banxia Decoction
- From:
Chinese Journal of Comparative Medicine
2024;34(11):26-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to evaluate the therapeutic effect of Gualou Xiebai Banxia Decoction(GXBD)on an atherosclerosis(AS)mouse model and explore its underlying mechanism.Methods Male ApoE-/-mice were fed a high-fat diet for 16 weeks to induce the AS model.Mice were divided into a model group,control group,GXBD treatment groups(at different doses),and a lipid-lowering drug group.Histological assessments(HE and Oil Red staining)were performed to observe arterial changes.MicroRNA(miRNA)sequencing was used to analyze the regulatory effect of GXBD.Western blot and immunofluorescence were applied to confirm key protein expression.Results GXBD significantly reduced plaque formation and regulated several AS-related miRNAs,notably impacting Epsin1 and FGFR1 protein expression.Western blot and immunofluorescence further validated these effects.Conclusions GXBD demonstrated a therapeutic effect on AS by regulating miR-3102-5p,miR-3547-5p,and miR-7080-5p,downregulating Epsin1,and upregulating FGFR1,thereby inhibiting endothelial-to-mesenchymal transition.
