Causal Relationship Between Immune Cell-mediated Intestinal Flora and Myasthenia Gravis
10.11969/j.issn.1673-548X.2025.08.013
- VernacularTitle:免疫细胞介导的肠道菌群与重症肌无力的因果关系
- Author:
Yang ZHAI
1
;
Xingwang PANG
;
Yan PANG
Author Information
1. 530001 南宁市第七人民医院脑病科
- Publication Type:Journal Article
- Keywords:
Gut microbiota;
Immune cells;
Myasthenia gravis;
Mendelian randomization;
Mediation analysis
- From:
Journal of Medical Research
2025;54(8):74-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the causal relationship between immune cell-mediated intestinal flora and myasthenia gravis(MG)by two-sample Mendelian randomization(MR)analysis.Methods The published Genome-Wide Association Study(GWAS)covering a total of 412 intestinal flora phenotypes and 731 immune cell phenotypes were used as exposure factors.The GWAS data of MG were obtained from the IEU Open GWAS project database.The sample size of the case group was 1278 cases,and the sample size of the control group was 33652 cases,containing 22357218 SNP.The subjects were all Europeans.Firstly,a two-sample MR analysis method was used to verify the causal relationship between intestinal flora and MG,immune cells and MG,intestinal flora and immune cells.Re-verse MR verifies the positive relationship.At the same time,sensitivity analysis was carried out,including heterogeneity test,level multi-effect test,leave-one-out sensitivity analysis and a series of verification analysis.Finally,the mediating effect of immune cells in the causal relationship between intestinal flora and MG was determined by two-step MR analysis.Results The causal relationship be-tween immune cell-mediated intestinal flora and MG was analyzed by inverse-variance weighted(IVW)method.The results showed that:Barnesiella(OR=1.835,95％CI:1.388-2.426),Barnesiella_intestinihominis(OR=1.961,95％CI:1.473-2.612),Eu-bacterium_biforme(OR=1.429,95％CI:1.155-1.766),Bacteroides_clarus(OR=1.220,95％CI:1.017-1.464),Roseburia_unclassified(OR=1.260,95％CI:1.053-1.509)were positively correlated with MG(P＜0.05).Streptococcus_parasanguinis(OR=0.715,95％CI:0.528-0.967)and Roseburia_intestinalis(OR=0.712,95％CI:0.517-0.982)were negatively correlated with MG(P＜0.05).The coefficient product method showed that the mediating effect of CD8dim％leukocyte in the inhibition of MG de-velopment by Roseburia_intestinalis accounted for 9.25％of the total effect.Conclusion Roseburia_intestinalis inhibits the occurrence and development of MG through CD8dim％leukocyte as an intermediary factor.This may provide new reference and basis for the preven-tion,treatment and rehabilitation of MG in the future.
