Surveillance and analysis of drug resistance molecular markers in Plasmodium vivax of imported cases in Chongqing
10.3969/j.issn.1002-2694.2025.00.081
- VernacularTitle:重庆市输入性间日疟原虫耐药分子标记物监测分析
- Author:
Yao XIANG
1
;
Yan TAN
;
Fei LUO
;
Jiaojiao CAI
;
Zhifeng LI
;
Jingru XU
;
Jingfu QIU
Author Information
1. 重庆医科大学公共卫生学院,重庆 400016;重庆市疾病预防控制中心(重庆市预防医学科学院),高致病性微生物重庆市重点实验室,重庆 400707
- Publication Type:Journal Article
- Keywords:
imported Plasmodium vivax;
drug resistance;
mutations;
Pvdhfr;
Pvdhps;
Pvmdr1;
Pvcrt-o;
Pvk12
- From:
Chinese Journal of Zoonoses
2025;41(7):726-734
- CountryChina
- Language:Chinese
-
Abstract:
This research aimed to comprehensively understand the prevalence of mutation in drug-resistant molecular markers of imported Plasmodium vivax in Chongqing,the Pvmdr1,Pvdhps,Pvdhfr,Pvcrt-o and Pvk12 genes of Plasmodium vivax were systematically analyzed.Blood samples were collected from imported Plasmodium vivax-infected patients in Chongqing between 2011 and 2022.The Pvmdr1,Pvdhps,Pvdhfr,Pvcrt-o,and Pvk12 genes were amplified and then sequenced to precisely evaluate gene mutations.Bioinformatics methods were employed to conduct in depth analysis of the mutation prevalence.Regarding the Pvdhfr gene,mutations at codons 50,57,58,61,99,117,and 199 were detected in 2.9%,23.5%,76.4%,23.53%,2.9%,82.3%,and 5.88%of the samples,respectively.The double-mutant haplotype S58R/S117N was the most prevalent,accounting for 50%,followed by the quadruple-mutant haplotype F57L/S58R/T61M/S117T,which accounted for 11.76%.Among the four types of tandem-repeat variations of Pvdhfr,the wild-type was the most common,and the insertion type was a novel discovery in this study.For the Pvdhps gene,the prevalence among mutation genotypes was relatively low.The single-mutant genotype was dominant,constituting 27.03%.The prevalence of Pvmdr1 mutations at codons 958 and 1076 was 100%and 89.19%,respectively.Among the 37 successfully sequenced samples,K10 insertion was detected in only 8 cases(22.22%).Notably,no non-synonymous mutations of Pvk12 were identified in this study.The cases in this study were imported from various countries of origin.Novel tandem-repeat variation tyres of Pvdhfr and new mutation sites of Pvdhps were identifide,thus enriching the mutation information of imported Plasmodium vivax resistance molecular markers in China.
