Transcriptomic analysis of differentially expressed genes in newly excysted juvenile Clonorchis sinensis cultured in vitro
10.3969/j.issn.1002-2694.2025.00.089
- VernacularTitle:基于转录组分析体外培养华支睾吸虫后尾蚴的实验研究差异表达基因
- Author:
Fengxi XIAN
1
;
Borong LI
;
Xueling DENG
;
Yuhong WU
;
Shitao LI
;
Yiqi JIANG
;
Siying ZHOU
;
Linrui LI
;
Zhanshuai WU
;
Zeli TANG
Author Information
1. 广西医科大学第一临床医学院,南宁 530021
- Publication Type:Journal Article
- Keywords:
Clonorchis sinensis;
metacercariae;
newly excysted juveniles;
differentially expressed genes;
transcriptome
- From:
Chinese Journal of Zoonoses
2025;41(7):718-725
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at investigating differentially expressed genes(DEGs)in Clonorchis sinensis(C.sinensis)meta-cercariae and newly excysted juveniles(NEJs)cultured in vitro for 1 hour or 3 hours,through transcriptomic analysis.Our objective was to explore the mechanisms underlying host invasion.Metacercariae were digested and isolated from Pseudorasbora parva infected with C.sinensis.The metacercariae excysted and developed into NEJs in vitro.Subsequently,the mRNA of metacercariae and NEJs cultured in vitro for 1 hour or 3 hours was extracted for transcriptomic sequencing analysis to screen for DEGs,and to conduct GO and KEGG analyses.A protein-protein interaction network(PPI)was constructed to identify hub genes.A total of 1 218 DEGs were de-tected.The main enriched GO terms of DEGs included transcription regulator activity and gated channel activity(primarily K+).The KEGG pathways significantly enriched in DEGs included cholesterol metabolism,lysosome,synthesis,secretion,and action of para-thyroid hormone.ZFAND4-2,BIRC6,and other genes were screened and identified as hub genes through PPI network analysis.Addi-tionally,abundant differential expression of cathepsin-related genes,including Cathepsin L and Cathepsin F,were observed before and after excystment in C.sinensis.Therefore,significant transcriptional level changes occurred in the metacercariae of C.sinensis be-fore and after excystation,and enrichment was observed primarily in signaling pathways,such as activation of growth and material me-tabolism,that regulate parasite growth and development.Meanwhile,biological events conducive to parasite invasion,migration,and adhesion were triggered.
