The effect of melatonin on the maturation level of oocytes and mitochondrial dynamics in mice exposed to benzophenone-3
10.3969/j.issn.1006-5725.2024.23.001
- VernacularTitle:褪黑素对二苯酮-3暴露小鼠卵母细胞成熟水平及线粒体动力学的影响
- Author:
Ruojin SHI
1
;
Yuying XIONG
;
Xueling ZHANG
;
Long JIN
;
Haiying ZHU
Author Information
1. 广东省产科重大疾病重点实验室,广东省妇产疾病临床医学研究中心,粤港澳母胎医学高校联合实验室,广州医科大学附属第三医院妇产科(广东 广州 510150)
- Publication Type:Journal Article
- Keywords:
melatonin;
benophenone-3;
mitochondrial dynamics;
oocyte maturation
- From:
The Journal of Practical Medicine
2024;40(23):3275-3283
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanisms of melatonin(MT)on improving oocyte quality in mice exposed to benzophenone-3(BP-3).Methods In this study,6～12-week-old female ICR mice were cultured in vitro in M16 culture,0.8 μmol/L BP-3 medium and 1 × 10-7 mol/L MT+0.8 μmol/L BP-3 mixed culture.Female ICR mice were randomly segregated into three groups:control,BP-3,and BMT.The control group received 0.5 mL of purified water,the BP-3 group was administered 0.5 mL of a 0.8 μmol/L BP-3 solution,and the BMT group received 0.5 ml of a combination of 0.8 μmol/L BP-3 and 15 mg/kg MT solution via gavage once daily for four weeks,to facilitate in vivo experimentation.Subsequently,the oocyte maturity rate,transcription levels and protein expression levels of mitochondrial dynamics-related genes Mfn1,Opa1,Fis1 and Drp1,mitochondrial membrane potential and spindle morphology were detected in the three groups to explore the rescue effect of MT on the mitochondria of BP-3-exposed mice.Results Compared to the control group,MT treatment markedly enhanced the transcription and protein levels of the mitochondrial fusion genes Mfn1 and Opa1 in oocytes,while concurrently down-regulating the mRNA and protein levels of the mitochondrial fission genes Fis1 and Drp1.Additionally,the BMT group exhibited significantly lower levels of ROS and abnormal spindle morphology in their oocytes compared to the BP-3 group,yet their mitochondrial membrane potential was notably elevated.Conclusion Physiological concentration of BP-3 exposure was toxic for reproduction,but the addition of appropriate concentrations of MT could significantly improve the mitochondrial dynamics and developmental potential of oocytes in BP-3-exposed mice.
