The effect of salidroside derivative pOBz on angiogenesis after ischemic stroke by regulating Notch signaling pathway
- VernacularTitle:红景天苷衍生物pOBz调控Notch信号通路对缺血性脑卒中后血管生成的作用
- Author:
Jing-quan CHEN
1
;
Yu-ting JIANG
;
Xue-rui ZHENG
;
Hui-ling WU
;
Qing-qing WU
;
Zheng-shuang YU
;
Wen-fang LAI
;
Gui-zhu HONG
Author Information
- Publication Type:Journal Article
- Keywords: salidroside derivative pOBz; ischemic stroke; platelet endothelial cell adhesion molecule; Notch signaling pathway; human umbilical vein endo-thelial cells; angiogenesis
- From: Chinese Pharmacological Bulletin 2025;41(12):2253-2259
- CountryChina
- Language:Chinese
- Abstract: Aim To study the effect of p-benzoyl sali-droside(pOBz)on angiogenesis after ischemic stroke and to explore the underlying mechanism.Methods The MCAO model was prepared by suture method.Rats were divided into four groups:sham,MCAO,pOBz administration,and edaravone positive control,treated for seven days.The mNSS was used to assess the neurological impairment.Western blotting was em-ployed to detect CD31,NICD,and Hes-1 protein ex-pression,while immunofluorescence staining was ap-plies to quantify CD31-positive cells in ischemic brain tissue.In vitro an OGD/R model was established in HUVECs.Following treatment with varying pOBz con-centrations(0.01,0.1,1 μmol·L-1),the CCK-8 as-say was uses to measure cell viability,and in vitro tube formation assay was utilized to evaluate angiogenesis.Western blotting was employed again to assess CD31,NICD and Hes-1 protein levels.To further elucidate the mechanism,HUVEC were treated with the Notch inhibitor DAPT prior to grouping and pOBz administra-tion,and the same parameters were evaluated.Results pOBz significantly reduced the mNSS score of MCAO rats,increased CD31-positive cell counts,and upregu-lated CD31,NICD,and Hes-1 protein expression(P＜0.01).In vitro results further showed that pOBz could dose-dependently increase the survival rate and angio-genesis ability of HUVEC induced by OGD/R,and promote CD31,NICD and Hes-1 proteins(P＜0.01),and Notch inhibitor DAPT could reverse the above effects of pOBz.Conclusion pOBz promotes angio-genesis in HUVEC,and its mechanism involves activa-tion of the Notch signaling pathway.
