Association between neutrophic extracellular trap marker(cell-free DNA)and mycoplasma pneumoniae pneumonia in children
10.3969/j.issn.1006-5725.2025.23.012
- VernacularTitle:中性粒细胞胞外诱捕网标记物游离DNA与儿童肺炎支原体肺炎的关系
- Author:
Xueni YANG
1
;
Yihang HU
1
;
Min JI
1
;
Yuqin LI
1
;
Hongyan LU
1
;
Ming CHANG
1
Author Information
1. 江苏大学附属医院儿科(江苏 镇江 212001)
- Publication Type:Journal Article
- Keywords:
mycoplasma pneumoniae pneumonia;
neutrophil extracellular traps;
C reactive protein;
interleukin-6
- From:
The Journal of Practical Medicine
2025;41(23):3711-3716
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study is to analyze the expression level of cell-free DNA(cf-DNA),a biomarker of neutrophil extracellular traps(NETs),in children with Mycoplasma pneumoniae pneumonia(MPP),and to explore the predictive efficacy of cf-DNA(as a marker of NETs)for the severity of MPP in these children.Methods A total of 115 children with MPP were prospectively selected as the MPP group.Based on the disease severity,the MPP group was categorized into the mild group(n=75)and the severe group(n=40).During the same period,50 healthy children undergoing physical examinations were selected as the control group.The levels of serum cf-DNA in the MPP group and the control group,as well as the levels of C-reactive protein(CRP),D-dimer,lactate dehydrogenase(LDH),interleukin-6(IL-6),interferon-γ(IFN-γ),and tumor necrosis factor-α(TNF-α)in the MPP group were detected.The differences in the levels of serum cf-DNA and related inflammatory factors among the groups were compared,and the role of serum cf-DNA in evaluating the severity of MPP was analyzed.Results The level of serum cf-DNA in children of the MPP group was notably higher than that in the control group,with a more significant elevation observed in the severe group(P<0.05).The levels of CRP,D-dimer,LDH,IL-6,IFN-γ,and TNF-α were all higher in the severe group than in the mild group(P<0.05).Multivariate logistic regression analysis showed that the increased levels of serum cf-DNA,CRP,and IL-6 were closely related to the severity of MPP(P<0.05).The results of receiver operating characteristic(ROC)curve analysis showed that the area under the curve(AUC)of the combination of serum cf-DNA,CRP,and IL-6 for predicting severe MPP was 0.981,which was higher than that of each index alone(P<0.05).Conclusions Serum cf-DNA(as a marker of NETs)is closely related to the severity of MPP in children.The combined detection of cf-DNA,CRP,and IL-6 is more beneficial for assessing the severity of MPP in children.
