Calumenin knockdown inhibits cell migration,invasion,and epithelial-mesenchy-mal transition in gastric cancer
10.13315/j.cnki.cjcep.2025.10.012
- VernacularTitle:敲低Calumenin抑制胃癌细胞迁移侵袭和上皮-间质转化
- Author:
Jiao LIU
1
;
Shan XU
;
Shuyao XIAO
;
Qiong LUO
;
Qian FU
;
Hui LING
Author Information
1. 长沙市第四医院(长沙市中西医结合医院)病理科,长沙 410006
- Publication Type:Journal Article
- Keywords:
gastric neoplasms;
Calumenin;
migration;
invasion;
epithelial-mesenchymal transition
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(10):1338-1344
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the effect of Calumenin(CALU)on migration and invasion ability of gastric cancer cells,as well as epithelial-mesenchymal transition(EMT).Methods The immunohistochemical experiments and Western blot were applied to evaluate the protein level of CALU in gastric cancer.After constructing a gastric canc-er cell line with low expression of CALU,CCK8 assay,wound-healing analysis and Transwell migration and invasion assays were performed to determine cell proliferation,Migration and invasion ability.Western blot was performed to an-alyse the effect of CALU knockdown on EMT molecules.Results CALU expression was significantly higher in gastric cancer tissues compared to normal gastric tissues(P＜0.05),and high CALU expression was significantly associated with TNM stage and lymph node metastasis(P＜0.05).Compared with GES-1 cells,the protein expression of CALU upregulated in gastric cancer cells(P＜0.05).CALU knockdown suppressed the proliferation,migration,invasion of BGC823 cells and SGC7901 cells(P＜0.05).Rescue experimental evidence showed that synonymous mutations of CALU could reverse the inhibitory effect of CALU knockdown on the proliferation,migration,and invasion ability of gastric cancer cells(P＜0.05).Knockdown of CALU resulted in the downregulation of vimentin and Snail expression,while E-cadherin and β-catenin expression were upregulated in human gastric cancer cells(P＜0.05).Conclusion CALU knockdown inhibits the proliferation,migration,invasion,and EMT of human gastric cancer cells.
