Clinical significance of miRNA-219 and calcium/calmodulin-dependent protein kinase Ⅱ γ subunit mRNA levels in peripheral blood of patients with depression
10.3760/cma.j.issn.1006-7884.2019.02.006
- VernacularTitle:抑郁症患者外周血miRNA-219和钙调蛋白激酶Ⅱγ亚基mRNA表达水平的临床意义
- Author:
Zhenghe YU
1
;
Yi LI
;
Jing LI
;
Mingfen SONG
;
Shengdong WANG
Author Information
1. 杭州市第七人民医院心身科 310013
- Publication Type:Journal Article
- Keywords:
Depressive disorder;
Receptors,N-methyl-D-aspartate;
Calcium-Calmodulin-dependent protein kinase type 2;
miRNA-219
- From:
Chinese Journal of Psychiatry
2019;52(2):134-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of miRNA-219 and γCaMK Ⅱ mRNA in the peripheral blood of the first-episode major depressive disorder (MDD) patients,and explore the relationship between these molecular level and MDD symptom.Methods Fourty-one first-episode major depressive disorder patients diagnosed with DSM-5 criteria and 31 heahy volunteers were included.The blood was collected in the fasting state and the expression levels of miRNA-219 and γCaMK Ⅱ mRNA was detected.The patients' severity of symptoms was assessed with HAMD17.Real-time PCR was used to detect the levels of miRNA-219 and γCaMK Ⅱ mRNA in peripheral blood leukocytes.The correlation between miRNA-219,γCaMK Ⅱ mRNA and HAMD17 was analyzed.Results The miRNA-219 levels of MDD group was significantly lower than that of control group (0.91 ±0.21 vs.1.80±0.24,t=2.753,P=0.0075) in peripheral blood,and the γCaMK Ⅱ mRNA levels of MDD group was significantly higher than that of control group (5.55±0.57 vs.2.16±0.20,t=4.970,P＜0.01).There was the positive correlation between γCaMK Ⅱ mRNA levels and the HAMD17 score of MDD (r=0.460,P=0.0025).Conclusion The levels of miRNA-219 in peripheral blood of MDD patients decreased,and the levels of γCaMK Ⅱ mRNA increased,suggesting that miRNA-219 and γCaMK Ⅱ may be related to the pathogenesis of depression.
