Porphyromonas gingivalis Promotes the Development of Esophageal Squamous Cell Carcinoma by Upregulating HuR to Suppress hsa_circ_0057552
10.13865/j.cnki.cjbmb.2025.09.1186
- VernacularTitle:牙龈卟啉单胞菌上调HuR抑制hsa_circ_0057552促进食管鳞癌发展
- Author:
Rui YANG
1
;
Bian-Li GU
;
Lin-Lin SHI
;
Shuo-Xuan LI
;
Yao-Wu LANG
;
Zhi-Xiang ZUO
;
She-Gan GAO
Author Information
1. 河南科技大学第一附属医院(临床医学院),肿瘤医院,河南省微生态与食管癌防治重点实验室,河南省肿瘤表观遗传重点实验室,河南洛阳 471003
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma(ESCC);
Porphyromonas gingivalis;
human antigen R(HuR);
hsa_circ_00057552
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(11):1678-1686
- CountryChina
- Language:Chinese
-
Abstract:
Recent studies have revealed a significant association between Porphyromonas gingivalis(P.gingivalis)infection and poor prognosis in esophageal squamous cell carcinoma(ESCC).Although cer-tain circular RNAs(circRNA)have been shown to suppress ESCC tumorigenesis and progression,their regulatory mechanisms in P.gingivalis infection-associated ESCC remain elusive.In this study,RT-qPCR analysis demonstrated that P.gingivalis infection downregulated hsa_circ_0057552 expression in ESCC cells and tissues in a time-and dose-dependent manner.Actinomycin D assays further confirmed that P.gingivalis infection reduced the RNA stability of hsa_circ_0057552 in ESCC cells(P＜0.05).Functional assays in vitro and a subcutaneous tumor xenograft model in vivo revealed that hsa_circ_0057552 overexpression significantly inhibited ESCC cell proliferation,migration,invasion,and tumor growth(P＜0.05).Additionally,PCR array screening combined with RT-qPCR and Western blotting in-dicated that P.gingivalis infection markedly upregulated human antigen R(HuR)expression at both RNA and protein levels(P＜0.05).Mechanistic investigations demonstrated that HuR knockdown signifi-cantly increased hsa_circ_0057552 expression(P＜0.01),whereas hsa_circ_0057552 overexpression had no regulatory effect on HuR.Finally,si-HuR treatment reversed the inhibitory effect of P.gingivalis on hsa_circ_0057552 transcription.This study demonstrated that P.gingivalis may promote the progression of ESCC through a novel mechanism involving the regulation of HuR/hsa_circ_0057552,thereby identif-ying a novel therapeutic target and molecular marker for P.gingivalis-associated ESCC.
