Correlation of POSTN and NECTIN-3 expression with clinicopathological features and prognosis in urothelial carcinoma
10.13315/j.cnki.cjcep.2025.11.012
- VernacularTitle:尿路上皮癌中POSTN、NECTIN-3的表达与临床病理特征及预后的相关性
- Author:
Xiaodie ZHOU
1
;
Kai CHENG
;
Jianjun WANG
;
Xuan WANG
;
Bo YU
;
Qunli SHI
;
Qiu RAO
;
Wei BAO
Author Information
1. 解放军东部战区总医院/南京大学医学院附属金陵医院,南京 210002
- Publication Type:Journal Article
- Keywords:
urothelial carcinoma;
clinicopathology;
HER2;
FISH;
prognosis
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(11):1485-1491
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the relationship between the expression of POSTN and NECTIN-3 in urothelial carcinoma(UC)and its clinicopathologic features and prognosis.Methods Clinical data of 115 UC patients were col-lected.EnVision two-step method was used for immunohistochemical analysis to determine the expression of POSTN and NECTIN-3 in UC and their correlation with clinicopathological features and prognosis.Transcriptomic data from TCGA database were used to analyze the correlation between the expression of POSTN and NECTIN-3 in bladder cancer and the pathological stage and prognosis of bladder cancer.qPCR and Western blot were used to detect the expression levels of POSTN and NECTIN-3 in tumor tissues and adjacent tissues of patients.Results TCGA database analysis showed that the expression level of NECTIN-3 in urinary bladder urothelial carcinoma(UBUC)was significantly lower than that in paracancer tissues,and POSTN expression was positively correlated with pathological stage.Prognostic a-nalysis showed that POSTN expression was negatively correlated with the overall survival of UBUC(P＞0.05),and NECTIN-3 expression was negatively correlated with disease-free survival of UBUC(P＞0.05).The experimental re-suits showed that patients with positive POSTN expression were more prone to perineural invasion[25 cases(86.2％)vs 4 cases(13.8％),P=0.019],vascular invasion[36 cases(83.7％)vs 7 cases(16.3％),P=0.007],and lymph node metastasis[24 cases(88.9％)vs 3 cases(11.1％),P=0.033].Additionally,the positive expression rate of POSTN in UBUC was significantly higher than that in upper tract urothelial carcinoma(UTUC)(75.0％vs 54.3％,P=0.028).Higher expression levels of POSTN and NECTIN-3 were associated with shorter overall survival,but the difference was not statistically significant(P＞0.05).Conclusion There is no significant correlation between NECTIN-3 and the clinicopathological features and prognosis of urothelial carcinoma,while the expression of POSTN is correlated with the invasive clinicopathological features of UC,which has certain suggestive significance for clinical stage and prognosis.
