Androgen receptor regulates oxidative stress and inhibits cardiomyocyte autophagy and apoptosis in diabetic cardiomyopathy rats
10.3969/j.issn.1000-4718.2025.11.008
- VernacularTitle:雄激素受体通过调控氧化应激抑制糖尿病心肌病大鼠心肌细胞自噬和凋亡
- Author:
Jianhui LI
1
;
Shuwei NING
Author Information
1. 许昌市中心医院病理科,河南 许昌 461000
- Publication Type:Journal Article
- Keywords:
diabetic cardiomyopathy;
androgen receptor;
oxidative stress;
autophagy;
apoptosis
- From:
Chinese Journal of Pathophysiology
2025;41(11):2144-2149
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role and mechanism of androgen receptor(AR)in autophagy and apopto-sis of cardiomyocytes in diabetic cardiomyopathy(DCM)rats.METHODS:The DCM rat model was established,the car-diac function was detected by echocardiography,the myocardial fibrosis was detected by hematoxylin-eosin and Masson staining,and the expression of AR was detected by Western blot.The H9c2 rat cardiomyocyte injury model treated with high glucose(HG)was constructed,and AR inhibitor and agonist were added.The cell viability was detected by CCK-8 assay,lactate dehydrogenase(LDH)levels were detected by LDH kit,and reactive oxygen species and apoptosis levels were detected by flow cytometry.The expression of AR,and autophagy-and apoptosis-related proteins was detected by Western blot.Apoptosis was observed by TUNEL staining.RESULTS:The expression level of AR was decreased in the myocardium of DCM rats and in H9c2 cardiomyocytes.The AR inhibitor reduced AR content,aggravated HG-induced oxi-dative stress,and enhanced the autophagy and apoptosis.The AR agonist increased AR content,inhibited HG-induced oxidative stress,and alleviated the autophagy and apoptosis.CONCLUSION:The AR regulates oxidative stress and re-duces the autophagy and apoptosis of cardiomyocytes in DCM rats,which may be a new target for the treatment of DCM.
