Identification and analysis of the host proteins interacting with the virulence-asso-ciated factor MGF300-4L of African swine fever virus
10.16303/j.cnki.1005-4545.2025.10.02
- VernacularTitle:与非洲猪瘟病毒毒力相关因子MGF300-4L相互作用的宿主蛋白鉴定与分析
- Author:
Jing LAN
1
;
Rui LUO
;
Ruojia HUANG
;
Zhanhao LU
;
Yuan SUN
;
Tao WANG
;
Yuying YANG
;
Huaji QIU
Author Information
1. 长江大学动物科学技术学院,湖北荆州 434023;中国农业科学院哈尔滨兽医研究所动物疫病防控全国重点实验室,黑龙江哈尔滨 150069
- Publication Type:Journal Article
- Keywords:
African swine fever virus;
MGF300-4L protein;
STAT1;
virulence-associated factor
- From:
Chinese Journal of Veterinary Science
2025;45(10):2102-2109
- CountryChina
- Language:Chinese
-
Abstract:
The MGF300-4L protein of African swine fever virus(ASFV),a virulence-associated fac-tor,degrades IKKβ through the chaperone-mediated autophagy and enhances the stability of IKBαto suppress the generation of IL-1β and TNF-α regulated by the NF-κB signaling pathway.To iden-tify the host proteins interacting with MGF300-4L,PK-15 cells were transfected with the eukary-otic plasmid expressing MGF300-4L and analyzed using immunoprecipitation-mass spectrometry(IP-MS)to identify the host proteins that interact with MGF300-4L.Additionally,gene ontology(GO)and KEGG pathway enrichment analyses were conducted.Furthermore,molecular docking a-nalysis,co-immunoprecipitation,and laser confocal microscopy assays were performed to validate the host proteins interacting with MGF300-4L.The IP-MS analysis identified 145 host proteins that potentially interact with MGF300-4L.Subsequent GO and KEGG pathway enrichment analy-ses revealed that these proteins are predominantly involved in metabolic,cellular,and innate immune responses.Through molecular docking prediction,co-immunoprecipitation assay,and laser confocal microscopy,we identified the interaction between MGF300-4L and STAT1.This study provides critical insights into the mechanisms underlying the interactions between MGF300-4L and the host proteins.
