Identification of porcine-derived circ_PIK3C2A and its effect on PEDV replication in host cells after infection
10.16303/j.cnki.1005-4545.2025.10.03
- VernacularTitle:猪源circ_PIK3C2A的鉴定及其对PEDV侵染宿主细胞后复制的影响
- Author:
Siqi JIA
1
;
Rongrong LIU
;
Yingjin CHAI
;
Xinxin HAN
;
Mingqing WEI
;
Tingting WU
;
Ying DING
;
Shaoxiu CHEN
;
Xingmei DENG
;
Hui ZHANG
Author Information
1. 石河子大学动物科技学院新疆生产建设兵团动物疾病防控重点实验室,新疆石河子 832003
- Publication Type:Journal Article
- Keywords:
circular RNA;
ssc_circ_PIK3C2A;
PEDV;
virus replication
- From:
Chinese Journal of Veterinary Science
2025;45(10):2110-2117
- CountryChina
- Language:Chinese
-
Abstract:
Circular RNA(circRNA),as a kind of non-coding RNA,regulates a variety of biological functions.To explore the effect of circRNA on PEDV replication in the host porcine intestinal epi-thelial cells,this study screened and analyzed the differentially expressed circRNAs by bioinforma-tic software in African Green Monkey renal cells(Vero-E6 cells)infected by porcine epidemic di-arrhea virus(PEDV),the differentially expressed circRNA ssc_circ_PIK3C2A was identified and the secondary structure was analyzed.PCR was used to identify the ssc_circ_PIK3C2A circRNA structure,the model of PEDV-infected IPEC-J2 cells was constructed,the TCID50 test was used to validate the viral titer of PEDV.The expression of circ_PIK3C2A was detected by qRT-PCR in IPEC-J2 infected by PEDV.circ_PIK3C2A qRT-PCR was performed to detect the expression of N gene of PEDV when ssc_circ_PIK3C2A was over-expressed in IPEC-J2 cells.The results showed that ssc_circ_PIK3C2 A is a porcine circular RNA with a typical circular structure,the virus titer of PEDV reached 10-6/mL after PEDV infected IPEC-J2 cells for 48 h,the expression of circ_PIK3C2A increased extremely(P＜0.01)at 6 h after PEDV-infection,with the extension of infec-tion time,its expression gradually decreased,and the expression was the lowest at 24 h,but there was no time-dependent trend.The expression of PEDV N gene decreased significantly when ssc_circ_PIK3C2A was over-expressed in IPEC-J2 cells.In conclusion,when PEDV infects IPEC-J2 cells,the expression of porcine circ_PIK3C2A decreases,and replication of PEDV increases signifi-cantly in IPEC-J2 cells.our result provides a basis for further study of the mechanism of circular RNA on PEDV replication and its physiological activities in host cells in the future.
