Screening of Effective Substances Targeting RSV-F Protein in Forsythia suspensa based on SPR Fishing Technology
10.11842/wst.20240918002
- VernacularTitle:基于SPR垂钓技术的连翘靶向RSV-F蛋白"功效物质"筛选
- Author:
Lin WANG
1
;
Ping WANG
;
Wen CAI
;
Xiaoyan DING
;
Yi WANG
;
Cheng WANG
;
Mengru ZHANG
;
Haitao DU
Author Information
1. 山东中医药大学药学院 济南 250355
- Publication Type:Journal Article
- Keywords:
Forsythia suspensa;
SPR fishing technology;
RSV-F protein;
Molecular docking;
Molecular dynamics simulation
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(5):1405-1416
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effective substances that specifically bind to RSV-F protein in the water extract of Forsythia suspensa,and verify its effect.Methods Surface plasmon resonance(SPR)affinity fishing technology was used to enrich,regenerate and recover the active components that specifically bind to RSV-F protein in the water extract of Forsythia suspensa.The recovered samples were analyzed and identified by UHPLC-MS/MS,and the active components that stably bind to F protein were screened by molecular docking,molecular dynamics simulation and SPR affinity detection,and then returned to animal experiments for pharmacodynamics verification.BALB/c mice were randomly divided into normal group,model group,positive control group,protocatechuic acid group and verbascoside group.In the experimental group,RSV was dripped into the nose to make models.Each drug group began to be given intragastric intervention on the day of modeling,and samples were taken after 4 days.HE staining was used to observe the pathological changes of lung tissue,and ELISA was used to detect the changes of inflammatory factors in lung tissue.Results A variety of active components in the aqueous extract of Forsythia suspensa could bind to RSV-F protein,among which the results of verbascoside and protocatechuic acid were more stable.In vivo pharmacodynamics experiments showed that the lung tissue of the model group had obvious pathological changes and the level of inflammatory factors increased,and the pathological state was improved after the intervention of verbascoside and protocatechuic acid compared with the normal group.Conclusion Forsythia suspensa can interfere with the expression of RSV-F protein through many active components such as verbascoside and protocatechuic acid,which may be the material basis of its anti-RSV.
