Molecular epidemiological survey of Giardia and Cryptosporidium in Ochotona curzoniae in Zoige County,Sichuan Province
10.3969/j.issn.1002-2694.2025.00.045
- VernacularTitle:若尔盖县高原鼠兔贾第虫及隐孢子虫分子流行病学调查
- Author:
Hong-xi CHEN
1
;
Yang XIANG
;
Ri-hong JIKE
;
Tian-xiang CHEN
;
Dong-bo YUAN
;
Liang-quan ZHU
;
Li-li HAO
Author Information
1. 西南民族大学畜牧兽医学院,成都 610041
- Publication Type:Journal Article
- Keywords:
Ochotona curzoniae;
Giardia lamblia;
Cryptosporidium;
nested PCR;
epidemiology
- From:
Chinese Journal of Zoonoses
2025;41(3):331-338
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at investigating infections with Giardia and Cryptosporidium in Ochotona curzoniae in Zoige County,Sichuan Province.O.curzoniae were captured in five townships of Zoige County(Dazhasi,Axi,Hongxing,Tangke,and Maixi)between March and December of 2023.DNA from the gastrointestinal contents was subjected to nested PCR to amplify Giardia bg,gdh,and tpi genes,and the Cryptosporidium SSU rRNA gene.The sequences of PCR-PCR products were analyzed and compared.Phylogenetic trees were constructed to determine the protozoa species and genotypes.A total of 114 O.curzoniae animals were captured,among which 44 samples showed bg gene positivity,and 14 samples showed gdh gene positivity for Giardia.The total detection rate was 43.9％(50/114),and two assemblages were detected(assem-blage E and a new assemblage tentatively termed assemblage OC1);the positivity rate for Cryptosporidium was 7.0％(8/114),and three new genotypes were observed.Mixed infection with Cryptosporidium and Giardia was present in some sam-ples,with a detection rate of 3.5％(4/114).Giardia lamblia and Giardia sp.(REG-1,REG-2)were prevalent in O.curzoni-ae in Zoige County in Sichuan province;assemblage E was the dominant assemblage,and the new assemblage OC1 was pres-ent;and Cryptosporidium sp.(REG-1,REG-2,and REG-3)were identified.In summary,future monitoring of Giardia and Cryptosporidium should be further strengthened in Zoige to provide detailed data for promoting local public health.
