An animal model of temporomandibular joint osteoarthritis established by perforation of the articular disc
10.3969/j.issn.1001-3733.2025.03.005
- VernacularTitle:通过关节盘穿孔建立颞下颌关节骨关节炎动物模型
- Author:
Zerou ZHANG
1
;
Dan JIN
;
Bingshuai JING
;
Rui REN
;
Mian ZHANG
;
Fuwei LIU
;
Yunpeng LI
Author Information
1. 710032 西安,口颌系统重建再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床研究中心,空军军医大学口腔医院口腔颌面外科
- Publication Type:Journal Article
- Keywords:
Temporomandibular joint;
Osteoarthritis;
Animal model;
Anatomy;
Cartilage
- From:
Journal of Practical Stomatology
2025;41(3):328-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to establish an animal model that accurately replicates the clinical symptoms and pathologi-cal changes of late-stage human TMJOA,with the goal of providing a standardized and reliable animal experimental method for sub-sequent research on the disease.Methods:Forty-eight male New Zealand rabbits aged 4 to 6 months were randomly divided into a model group and a sham group.The animals in the model group underwent bilateral temporomandibular joint disc perforation surgery,while the animals in the sham group underwent a sham surgery.The modeling effects were assessed at 4,8,and 12 weeks post-surgery using nociceptive behavior assessments,passive mouth opening measurements,histological analysis(HE,Safranin O-fast green staining),immunohistochemistry,immunofluorescence staining,and magnetic resonance imaging(MRI).Results:Animals in the model group exhibited distinct TMJOA symptoms,including joint pain and restricted mouth opening.Histologically,typical osteo-arthritis changes were evident.The Mankin osteoarthritis score was significantly higher(P＜O.05).Conclusion:The TMJ disc perfo-ration model resmbled the same clinical manifestations and pathological changes seen in human TMJOA.
