The role of the Hippo signaling pathway in the epithelial-mesenchymal transition of triple-negative breast cancer cells
10.3760/cma.j.cn431274-20240507-00751
- VernacularTitle:Hippo信号通路在三阴性乳腺癌细胞上皮-间质转化中的作用
- Author:
Yuanyuan HUO
1
;
Xiangyun HAO
1
;
Caiping JING
1
Author Information
1. 延安市人民医院病理科，延安 716000
- Publication Type:Journal Article
- Keywords:
Triple negative breast cancer;
Hippo signaling pathway;
Epithelial-mesenchymal transition
- From:
Journal of Chinese Physician
2025;27(5):709-713
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the role of the Hippo signaling pathway in epithelial-mesenchymal transition (EMT) of triple-negative breast cancer (TNBC) cells.Methods:70 patients with TNBC admitted to the Yan ′an People′s Hospital from April 2021 to September 2023 were selected. Before treatment, real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression levels of Hippo signaling pathway-related indicators (Yap, ta Z, mst 1) in peripheral blood, and the patients were treated with the TP regimen for 21 days. After the treatment, puncture biopsy was performed on the tumor tissues of the patients. They were divided into the transformation group and the non-transformation group based on whether EMT occurred. The influencing factors of EMT in tumor cells of TNBC patients were analyzed, and the value of the Hippo signaling pathway in predicting EMT in tumor cells of TNBC patients was analyzed.Results:After treatment, 18 cases (25.71%) of 70 patients with TNBC developed EMT. The proportion of tumor node metastasis (TNM) stage Ⅳ in the transformation group was (13/18), the level of Yap mRNA was (4.82±0.63), the level of taZ mRNA was (2.93±0.41), and the mst 1 mRNA level (3.09±0.51) and the long diameter of the tumor [(5.95±0.77)cm] were both higher than those in the non-transformation group [44.23%(23/52), (3.84±0.59), (2.34±0.39), (2.45±0.47), (4.14±0.63)cm] (all P<0.05). Binary logistic regression analysis revealed TNM stage Ⅳ [ OR=4.768, 95% CI(1.630, 13.951)], Yap mRNA [ OR=5.675, 95% CI(1.940, 16.602)], and taZ mRNA [ OR=5.124 95% CI(1.751, 14.992)], mst 1 mRNA [ OR=5.557, 95% CI(1.899, 16.257)], tumor long diameter [ OR=3.850, 95% CI(1.316, 11.263)] was an influencing factor for the occurrence of EMT in tumor cells of TNBC patients (all P<0.05). After analysis by the receiver operating characteristic (ROC) curve, the combined sensitivity of TNM stage Ⅳ, Yap mRNA, taZ mRNA, mst 1 mRNA, and tumor long diameter in predicting EMT in tumor cells of TNBC patients was 66.67%, 72.22%, 77.78%, 72.22%, 77.78%, and 88.89%, respectively. The specificities were 69.23%, 71.15%, 76.92%, 73.08%, 75.00% and 92.31% respectively. The combined prediction of TNM stage Ⅳ, Yap mRNA, taZ mRNA, mst 1 mRNA, and tumor long diameter in tumor cells of TNBC patients had a relatively high value (all P<0.05). Conclusions:The combined detection of TNM stage Ⅳ, Yap mRNA, taZ mRNA, mst 1 mRNA, and tumor long diameter can better predict the occurrence of EMT in tumor cells of patients with TNBC.
