Ratanasampil reduced levels of Aβ1-40 and Aβ1-42 and Aβ42/Aβ40 ratio in brains of Tg2576 mouse model of Alzheimer's disease
10.3760/cma.j.issn.1006-7884.2010.02.003
- VernacularTitle:藏药七十味珍珠丸对阿尔茨海默病小鼠模型脑组织Aβ表达的影响
- Author:
Ai-qin ZHU
1
;
Yi-de CHU
;
Qiao-xin LI
;
Collin L MASTERS
Author Information
1. 青海省人民医院老年医学研究所,西宁,810007
- Publication Type:Journal Article
- Keywords:
Tibetan drugs;
Alzheimer disease;
Mice,transgenic;
Amyloid bata-protein;
Open field activity and Y-maze tests
- From:
Chinese Journal of Psychiatry
2010;43(2):69-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ratanasampil (RNSP) which is traditional Chinese Tibet-Medicines on the β-amyloid peptide as a therapeutic target in Alzheimer's disease.Methods Thirteen female mice of 13-14 months old with Tg2576 transgene and ten BL6 × SJL mice as control were used in drug test All mice sacrificed after 8 weeks of RNSP treatment at 15-16 months of age.Open field activity and Y-maze tests were performed for animal behavioral change and memory ability.The β-amyloid peptides (Aβ1-40 and Aβ1-42) and β-amyloid precursor protein (APP) in Tg2576 mice brain were measured with western blot and enzyme linked immunosorbent assay (ELISA).Immunostaining with 1 E8 ( 1: 25 ) was carried out on brain sections of RNSP and vehicle-treated mice.Results The training times of Y-maze test decreased in RNSP-treated Tg2576 mice (P ＜0.01 ).After 2 months of RNSP treatment, a decrease in open field behavior was seen in Tg2576 mice ( P ＜ 0.05 ).The level of Aβ1-40 and Aβ1-42 in the brain was significantly decreased after RNSP treatment ( P ＜ 0.05 ).The Aβ42/Aβ40 ratio was significantly decreased after RNSP treatment as compared to vehicle-treated mice ( P ＜ 0.05 ) .But levels of APP in brain unchanged.Preliminary plaque counting of the sections showed reduced plaque numbers in the drug-treated mice in brain.Conclusions These results suggest that ratanasampil may reduce β-amyloid peptide production in brain to improve the learning and memory ability of Tg2576 mice of Alzheimer's disease.
