Cloning,bioinformatics analysis,expression and localization of APOD in bactrian camel epididymis
10.16303/j.cnki.1005-4545.2025.04.18
- VernacularTitle:双峰驼APOD的克隆、生物信息学分析及在附睾中的表达与定位
- Author:
Aili CUI
1
;
Wenjing WANG
;
Xue HUANG
;
Qiu YAN
;
Tianan LI
;
Jinghong NAN
;
Yong ZHANG
;
Xingxu ZHAO
;
Qi WANG
Author Information
1. 甘肃农业大学动物医学院,甘肃兰州 730070
- Publication Type:Journal Article
- Keywords:
bactrian camel;
estrus;
anestrus;
epididymis;
APOD
- From:
Chinese Journal of Veterinary Science
2025;45(4):752-759
- CountryChina
- Language:Chinese
-
Abstract:
Apolipoprotein D(APOD)is a protein that is widely present in animal tissues and is in-volved in the reproductive regulation of the body.In order to investigate the expression regularity of APOD in bactrian camel epididymis and its regulation effect on sperm maturation,this study took the epididymis of bactrian camel during estrus and anestrus as materials,and first cloned the complete sequence of APOD coding sequence(CDS)region.The physicochemical properties of AP-OD were analyzed by ProParam,SOPMA,SWISS-MODEL and MEGA7.0 software.Meanwhile,the expression and distribution of APOD in epididymis were detected by qRT-PCR,Western blot and IHC.The cloning results showed that:the length of the CDS region of APOD gene was 624 bp,encoding 207 amino acids.The APOD sequence of Bactrian camel was highly conserved with the nucleotide and amino acid sequence of alpaca,and the homology of APOD sequence with elk was the lowest.The results of qRT-PCR showed that the mRNA levels of APOD in the head,body and tail of epididymis in estrus were significantly higher than those in estrus(P＜0.01).Western blot results showed that the APOD protein expression and mRNA expression trend was similar in the head and body of the epididymis during anestrus,but the APOD expression level in the tail of the epididymis during anestrus was opposite to the mRNA expression level(P＜0.05).The results of H&E and IHC showed that there were significant differences in epididymal tissue between estrus and anestrus.In addition,APOD showed positive reactions in epididymal epithelial cells,smooth muscle cells,sperm and connective tissue to varying degrees,suggesting that APOD may be in-volved in the maturation of sperm during estrus and anestrus,providing evidence for further explo-ring the regulatory mechanism of APOD's involvement in seasonal estrus.
