Exploring mechanism of Lycium barbarum polysaccharides in preventing inflam-matory bowel disease in chicks based on network pharmacology
10.16303/j.cnki.1005-4545.2025.04.22
- VernacularTitle:基于网络药理学探讨枸杞多糖预防蛋雏鸡炎症性肠病的作用机制
- Author:
Nana GAO
1
;
Yang LI
;
Fenglong CHEN
;
Xu LIU
;
Heping BAI
;
Qian LI
;
Xiaodan WANG
Author Information
1. 河北农业大学 中兽医学院,河北保定 071000
- Publication Type:Journal Article
- Keywords:
Wolfberry;
Lycium barbarum polysaccharides;
inflammatory bowel disease;
chicks;
net-work pharmacology;
molecular docking
- From:
Chinese Journal of Veterinary Science
2025;45(4):794-806
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to explore protective effects of Lycium barbarum polysaccharides(LBP)on intestinal damage caused by lipopolysaccharide(LPS)-induced inflammatory bowel disease(IBD)in chicks.Network pharmacology was initially employed to determine the target proteins of wolfberry in the prevention and treatment of IBD.Following this,protein-protein interaction analy-sis,GO and KEGG pathway enrichment analysis,and molecular docking studies were conducted.Subsequently,an animal study was conducted:a total of 100 one-day-old male Hy-line brown lay-ing hens were randomly divided into five groups:a blank control group(CON),an LPS treatment group(LPS),a low-dose LBP group(LPS+LBP 0.25 g/L,L-LBP),a medium-dose LBP group(LPS+LBP 0.5 g/L,M-LBP),and a high-dose LBP group(LPS+LBP 1 g/L,H-LBP).Upon reac-hing 21 days old,duodenal,jejunal,ileal,and cecal tissues were collected to determine SOD and GSH-Px levels.Furthermore,the mRNA expression levels of TNF-α,AKT1,IL-6,IL-1β and TP53 in the intestinal tissues were measured using quantitative real-time PCR.The results demonstrated that network pharmacology identified 45 active ingredients in wolfberry that target 116 key protein sites,including TNF,AKT1 and IL6.The primary objectives focus on signaling pathways including AGE-RAGE,IL-17,TNF,HIF-1,and NF-κB.Molecular docking showed excellent ligand-receptor docking scores,with stable binding facilitated by hydrogen bonds and hydrophobic interactions.Compared to the LPS group,the 0.5 g/L LBP exhibited notably higher levels of SOD and T-AOC.In comparison with the LPS group,the medium and high-dose LBP experimental groups showed notably decreased the mRNA expressions of TNF-α,AKT1,IL-6,and IL-1β,while TP53 mRNA expression was significantly upregulated(P＜0.01).In summary,wolfberry exerts preventive and therapeutic effects on IBD through a multi-component,multi-target,and multi-pathway mecha-nism.
