Effect of peripheral blood FGF21 levels on glucose uptake and utilization by neu-trophils in perinatal dairy cows
10.16303/j.cnki.1005-4545.2025.04.16
- VernacularTitle:外周血FGF21水平对围产期奶牛中性粒细胞摄取和利用葡萄糖的影响
- Author:
Yan REN
1
;
Kexin WANG
1
;
Xinwei LI
1
;
Yuxiang SONG
1
;
Guowen LIU
1
Author Information
1. 吉林大学动物医学学院,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
perinatal cows;
neutrophils;
glucose metabolism;
FGF21
- From:
Chinese Journal of Veterinary Science
2025;45(4):738-744
- CountryChina
- Language:Chinese
-
Abstract:
The decrease in blood glucose in perinatal dairy cows affects the energy supply and im-mune function of peripheral blood neutrophils(PMN).Fibroblast growth factor 21(FGF21)is an important regulator of glucose metabolism,and serum FGF21 levels in some perinatal cows are significantly elevated after farrowing.In order to explore the effect of FGF21 on PMN glucose ho-meostasis in perinatal dairy cows,the cows were divided into high FGF21 group(high FGF21,n=8,FGF21＞800 ng/L)and low FGF21 group(low FGF21,n=8,FGF21＜200 ng/L)within 3 weeks postpartum.The results showed that compared with the low FGF21 group,the glucose up-take of PMN and the mRNA expression of glucose transporters SLC2A1 and SLC2A3 were signifi-cantly increased in the high FGF21 group.The activities of phosphofructokinase 1(PFK1)and glu-cose-6-phosphate dehydrogenase(G6PDH)were significantly increased,and the activity of glyco-gen synthase(GCS)was significantly decreased in PMN in the high FGF21 group.The lactate con-tent and ATP content of PMN were significantly increased,and the mRNA and protein expressions of hexokinase(HK2)and PFK1 were significantly increased in the high FGF21 group.These re-sults indicated that the uptake and utilization of glucose by PMN in perinatal peripheral blood FGF21 increased to ensure the ATP supply of PMN,which provided a theoretical basis for the pro-posal of a new strategy to alleviate immunosuppression in perinatal dairy cows.
