Expression and distribution of FDC and Tfh in mice induced by foot-and-mouth disease virus-like particles
10.16303/j.cnki.1005-4545.2025.04.04
- VernacularTitle:口蹄疫病毒样颗粒诱导的小鼠FDC和Tfh的表达与分布
- Author:
Ya SU
1
;
Jiahuan LIU
1
;
Junjuan ZHANG
1
;
Limin LI
1
Author Information
1. 河北农业大学动物医学院,河北保定 071000
- Publication Type:Journal Article
- Keywords:
virus-like particles;
follicular dendritic cells;
follicular helper T cells;
immunohistochem-istry
- From:
Chinese Journal of Veterinary Science
2025;45(4):648-655
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression and distribution of follicular dendritic cell(FDC)and follic-ular helper T cells(Tfh)in mouse spleen and lymph nodes induced by foot-and-mouth virus-like particles(FMDV-VLPs),the recombinant pCMV-HA-HBcAg-VP1-VP4 plasmid was transfected into CHO-K1 cells to prepare FMDV-VLPs,and the recombinant FMDV VP1-VP4 protein was expressed and purified through a prokaryotic expression system.BALB/c mice were subcutaneous-ly immunized with FMDV-VLPs(VLP group)and recombinant FMDV VP1-VP4 protein(VP1-VP4 group),and PBS control group(PBS group)was set up.At 21 d post primary immunization(dpi),booster immunization was carried out.Spleen,shoulder lymph nodes,and submandibular lymph nodes were collected at 7,28,42,72 and 102 dpi,and frozen sections were prepared.Immu-nohistochemical staining was used to observe the expression and distribution of FDC and Tfh.The results showed that the number of FDC and Tfh cells in the VLP group in the spleen and lymph nodes showed a gradual increase from 7 to 42 dpi,and gradually decreased to non-immune levels starting from 72 dpi.The results indicate that FMDV-VLPs can induce the formation of GC in the spleen and lymph nodes,and the number of FDC and Tfh cells in GC can last for at least 42 d.This study provides a theoretical basis for the study of the immune memory response mechanism of B cells induced by FMDV virus-like particles.
