Mechanism of adipose mesenchymal stem cell exosomes inhibiting atopic dermatitis
- VernacularTitle:脂肪间充质干细胞外泌体抑制特应性皮炎的作用机制
- Author:
Jia-qi BI
1
;
Zhao WANG
;
Bing-kun WANG
;
Chun-yan SUN
;
Ya SUN
;
Xiao-tong CUI
;
Xin PANG
;
Xiao-yu WANG
;
Jie-qiong WANG
Author Information
- Publication Type:Journal Article
- Keywords: adipose mesenchymal stem cells; exo-some; atopic dermatitis; epidermal barrier; allergic diseases; cell extraction
- From: Chinese Pharmacological Bulletin 2025;41(6):1148-1157
- CountryChina
- Language:Chinese
- Abstract: Aim To study the mechanism of adipose mesenchymal stem cell exosomes(ASC-exo)inhibition of fluorescein isothiocyanate(FITC)-induced atopic dermatitis(AD).Methods The mouse age,extrac-tion method,and the concentration of a solution of typeⅠ collagen enzyme and other conditions were compared to study the effects on the morphology and quantity of adipose mesenchymal stem cells(ASCs)after extrac-ted.FITC-induced mouse model in vivo was estab-lished and different doses of ASC-exo were given to measure ear thickness,ear weight and ear scratching times of mice.HE staining was used to observe the pathological changes of ear tissue of mice.The non-toxicity of ASC-exo was detected.IgE,IL-5,IL-13 and other cytokines were detected by ELISA.The gene ex-pressions of TSLP,IL-33,occludin,Claudin-1(CLDN-1)and E-cadherin were detected by RT-qPCR.The protein expression was detected by immunohistochemis-try.Results An efficient method for extracting ASCs was established.Compared with the blank group,mice in the model group showed obvious AD symptoms.Compared with the model group,ASC-exo administra-tion group significantly reduced the number of ear scratches,epidermal thickening,inflammatory cell infil-tration and the secretion of Th2 cytokines IL-5 and IL-13.Meanwhile,ASC-exo administration group signifi-cantly increased the expression of structural proteins CLDN-1 and occludin in epithelial cells and decreased the expression of TSLP and IL-33.Conclusions ASC-exo can significantly improve Th2 skin inflamma-tion in AD mice,and its mechanism may be through in-creasing the expression of tight junction proteins and adhesion link protein in epithelial cells,repairing the skin barrier,and inhibiting the key promoters of allergy TSLP and IL-33.
