Research on coagulation effect of cold atmospheric plasma jet device and its mechanism of action
10.19745/j.1003-8868.2025101
- VernacularTitle:大气压冷等离子体射流装置的凝血作用及其机制初步研究
- Author:
Yan LI
1
;
Hong-ye ZHENG
;
Ao-xi XU
;
Ya-jun ZHAO
;
Shan-shan JIN
;
Xu ZHANG
;
Yu-fan WEI
;
Yi-heng ZHANG
;
Li ZHU
;
Xi-ru LI
Author Information
1. 解放军医学院,北京 100853;解放军总医院第一医学中心普通外科,北京 100853
- Publication Type:Journal Article
- Keywords:
cold atmospheric plasma;
plasma jet;
coagulation device;
procoagulant and hemostatic;
coagulation mechanism
- From:
Chinese Medical Equipment Journal
2025;46(6):20-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the coagulation effect of a cold atmospheric plasma(CAP)jet device with helium as the working gas and to study its coagulation mechanism preliminarily.Methods A CAP jet device treatment group,a helium airflow treatment group,a hot air treatment group(60℃)and a natural coagulation group were formed according to the treatment modes of the blood samples,with 10 μL of blood samples involved in each group,in order to validate the coagulation effect of the CAP jet device in vitro;the coagulation mechanism of the CAP jet device was explored by its application to the treatment of anticoagulated whole blood,platelet-rich plasma and platelet-depleted plasma;the coagulation effect of the CAP jet device in vivo was verified with a mouse liver punctate hemorrhage model and a rabbit mesenteric hemorrhage model.Results The CAP jet device can significantly accelerate the coagulation of anticoagulated blood droplets,and the coagulation time of anticoagulated blood droplets in the CAP jet device-treated group was shortened from 28 min in the natural coagulation group to(23±1.56)s,with the difference statistically significant(P<0.05),and the CAP jet device treatment group gained advantages significantly over the helium airflow treatment group(P<0.05)and the hot air(60℃)treatment group(P<0.05)in coagulation-promoting effect;the procoagulant effect of the CAP jet device rose with the increase of platelet content in blood droplets,and the coagulation effect of platelet-rich blood droplets was significantly better than that of whole blood(P<0.05),while no coagulation was observed in platelet-poor droplets.The CAP jet device could rapidly stop hemostasis of punctate hemorrhage in mouse liver and mesenteric hemorrhage in rabbits without delayed hemorrhage occurring within 10 min,and no obvious structural abnormality of the liver and thermal damage of the tissue were found microscopically.Conclusion The CAP jet device plays procoagulant and hemostatic effects in vivo and in vitro,and its effect is not dependent on temperature and airflow evaporation effects and is considered to be related to platelet activation,with low thermal damage to living tissue.[Chinese Medical Equipment Journal,2025,46(6):20-27]
