Curcumin inhibits hydrogen peroxide induced pyroptosis of RSC96 cells through NLRP3/caspase-1/GSDMD pathway
10.16557/j.cnki.1000-7547.2024.05.013
- VernacularTitle:姜黄素通过NLRP3/caspase-1/GSDMD通路抑制过氧化氢诱导的RSC96细胞焦亡
- Author:
Ming LI
1
;
Si ZHENG
1
;
Jiayin WANG
1
;
Qing ZHU
1
;
Longju CHEN
1
Author Information
1. 湖北民族大学:风湿性疾病发生与干预湖北省重点实验室,恩施 445000;湖北民族大学:医学部解剖教研室,恩施 445000
- Publication Type:Journal Article
- Keywords:
curcumin;
hydrogen peroxide(H2O2);
pyroptosis;
NLRP3;
caspase-1;
GSDMD;
Schwann cells
- From:
Chinese Journal of Neuroanatomy
2024;40(5):619-625
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect and mechanism of curcumin on the rat Schwann cell derived cell line RSC96 cells pyroptosis induced by hydrogen peroxide.Methods:The rat Schwann cell derived cell line RSC96 cells were selected and treated with hydrogen peroxide to establish the peripheral nerve oxidative injury mod-el,curcumin intervention was also given.Cell viability was detected by CCK-8 kit.Lactate dehydrogenase(LDH)kit was used to detect the LDH leakage rate.The level of IL-1 β was detected by enzyme linked immunosorbent assay(ELISA).The protein expression levels of NLRP3,caspase-1,cleaved caspase-1,GSDMD,GSDMD-N and IL-1 βwere detected by Western Blot.Results:Cur significantly increased the viability of RSC96 cells treated with H2O2,decreased LDH leakage rate,down-regulated IL-1 β secretion,and inhibited the protein expression of NLRP3,caspase-1,cleaved caspase-1,GSDMD,GSDMD-N,and IL-1 β.Conclusion:Curcumin can inhibit hydrogen peroxide-induced pyroptosis of RSC96 cells through the NLRP3/caspase-1/GSDMD signaling pathway,thus exerting neuroprotective effect.
