Isolation,identification,homology and biological characteristics of Escherichia co-li from chicken
10.16303/j.cnki.1005-4545.2025.05.09
- VernacularTitle:不同来源鸡源大肠杆菌的分离鉴定、分子分型及耐药性致病性分析
- Author:
Jiayu WANG
1
;
Xiaojing HAO
;
Meiling SUN
;
Wenhua LIU
Author Information
1. 青岛农业大学动物医学院,山东青岛 266109
- Publication Type:Journal Article
- Keywords:
avian pathogenic E.coli;
pulsed field gel electrophoresis(PFGE);
homology;
biological characteristic
- From:
Chinese Journal of Veterinary Science
2025;45(5):948-953
- CountryChina
- Language:Chinese
-
Abstract:
In order to understand the infection status of Escherichia coli(E.coli)in different popu-lations of chickens,E.coli was isolated and identified from 1-day-old chickens,dead embryos,clini-cal dead chickens from 3 hatcheries and 9 broiler farms in Yantai area,and 1 950 samples from e-liminated breeder chickens;the homology of the isolated strains was analyzed by pulsed field gel e-lectrophoresis;virulence genes and serotypes were identified by PCR;pathogenicity was determined by challenge chicken embryos.Drug resistance of the strain was determined by drug sensitivity test.Results showed that a total of 116 strains of E.coli were isolated,of which 97 strains were successfully classified into 66 band types,15 virulence genes and 5 serotype were detected by PCR,and 56,32 and 28 strains were divided into high,medium and low pathogenic strains by chicken embryo challenge test.Results indicated that E.coli from clinically dead chickens and eliminated breeder chickens had higher pathogenicity and drug resistance.This study provides a reference ba-sis for the prevention and control of colibacillosis in chicken farms.
