Epidemiological investigation of PCV2 and distribution of swine farms in Henan province 2022-2023
10.16303/j.cnki.1005-4545.2025.05.06
- VernacularTitle:2022-2023年河南省猪圆环病毒2型流行病学调查及其在猪场内的分布
- Author:
Zhenkun LI
1
;
Jin YUAN
;
Hao LU
;
Jinchao XING
;
Yang LI
;
Fangting DONG
;
Menglong XU
;
Yue ZHANG
;
Zhanyong WEI
Author Information
1. 河南农业大学动物医学院,河南 郑州 450046;河南省动物性食品病原监测实验室,河南 郑州 450046;动物病原与生物安全教育部重点实验室,河南 郑州 450046
- Publication Type:Journal Article
- Keywords:
porcine circovirus type 2;
epidemiology;
ORF2 gene;
farm distribution
- From:
Chinese Journal of Veterinary Science
2025;45(5):926-933
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the epidemic variation of porcine circovirus type2(PCV2)and its distribution in swine farms in Henan Province from 2022 to 2023.In this study,1 206 pig blood samples from 18 urban scale swine farms,and 318 pigs and environmental samples from 2 PCV2-positive swine farms in Henan Province were detected by fluorescence quantitative PCR.ORF2 gene sequencing and genetic evolution analysis were performed on the positive samples.The results showed that the positive rate of PCV2 was 7.05％(85/1 206)in Henan Province,11.15％in western Henan Province and 3.03％in southern Henan Province.The positive rate of PCV2 in spring was 11.60％(67/576).A total of 31 ORF2 gene sequences were obtained,of which 23 were PCV2d(74.19％),6 were PCV2a(19.35％),one was PCV2b(3.23％)and one was PCV2c(3.23％).The results of amino acid sequence comparison showed that the mutation sites mainly concentrated on amino acids at positions 40-68 and 180-209.The total positive rate of pig farm sam-ples was 37.42％(119/318),and the positive rate of pig samples from different types of pig houses was 60.71％(34/56),45.83％(11/24),35.09％(20/57),and 33.33(3/9)in order from high to low.The positive rate of environmental samples from high to low was 56.36％(31/55)in feeding system,46.67％(7/15)in feces system,12.50(1/8)in decontamination area,11.11％(2/18)in living area,and no PCV2 was detected in ventilation system.The highest positive rate was 45.00％(9/20)for the tools in the house,7.14％(1/14)for the surface and sole of the clothes after leaving the house,and no PCV2 was detected on the surface and sole of the clothes before entering the house.In this study,the epidemiological investigation of PCV2 in Henan Province and its distribu-tion in swine farms were carried out,and the main circulating strains,seasons,regions and gene mutations of PCV2 in H enan Province were identified.The distribution and transmission routes of PCV2 in positive pig farms were preliminarily analyzed and summarized,which provided data basis and reference for effective prevention and control of PCV and the establishment of accurate bio-safety prevention and control measures.
