Characterization of lipid droplet metabolism in hepatocytes in db/db mice
10.3969/j.issn.1006-6187.2024.12.011
- VernacularTitle:db/db小鼠肝细胞脂滴代谢特征的研究
- Author:
Wen SUN
1
;
Ye XU
;
Hongjian XIAO
;
Wanyu FENG
;
Xinmeng YUAN
;
Mengqi XIANG
;
Jiao LU
;
Qiang TANG
;
Yuan ZHANG
Author Information
1. 210014 南京体育学院运动健康学院
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,type 2;
Hepatic lipid deposition;
Lipid droplet metabolism;
Lipolysis;
Lipophagy
- From:
Chinese Journal of Diabetes
2024;32(12):932-940
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the hepatic lipid droplet metabolism in normal BKS-db/m mice and BKS-db/db mice,a model of type 2 diabetes mellitus (T2DM),and to investigate the characteristics of hepatic lipid droplet metabolism and the molecular mechanisms underlying the imbalance of metabolic homeostasis at the level of lipid droplet synthesis and lipid droplet catabolism in db/db mice. Methods Eight SPF 8 weeks BKS-db/m and 8 BKS-db/db mice were used in this study. The lipid metabolism level was tested. Oil red and HE staining was used to evaluate the degree of liver lipid deposition,and immunofluorescence staining and Western blot was used to observe the level of hepatic lipid droplet catabolism and metabolism. Results The body weight,liver weight,liver index,FPG,AUC of the OGTT,AUC of the ITT,TG,TC,HDL-C,LDL-C,FFA and SREBP1 positive fluorescence area and nuclear translocation were higher in db/db group than in db/m group (P<0.05). The lipophagia level of primary hepatocytes was higher in db/db group than in db/m group (P<0.05). Compared with the db/m group,the expressions of SREBP1,SCD1,PLIN2,LC3,LAMP2A and ATGL protein increased(P<0.05),while the expression of p-ACC/ACC protein decreased in the db/db group(P<0.05). Conclusions Compared with normal mice,T2DM mice had dyslipidemiaand marked hepatocellular lipid deposition.Although lipid droplet lipolysis and lipophagy were enhanced and catabolism was significant,the lipid droplet metabolism was in a state of imbalance between synthesis and catabolism,and the synthesis of lipid droplets was greater than that of catabolism,which led to the aggregation of hepatocellular lipids.
