Antibacterial mechanism of allicin against carbapenem-resistant Klebsiella pneumoniae based on gene cluster analysis
10.11816/cn.ni.2025-246920
- VernacularTitle:基于基因聚类分析的大蒜素对耐碳青霉烯类肺炎克雷伯菌抑菌机制
- Author:
Fanglin GAO
1
;
Haitao RUAN
1
;
Wenfang ZHAN
1
;
Qing LIN
1
Author Information
1. 福建中医药大学附属人民医院检验科,福建 福州 350004
- Publication Type:Journal Article
- Keywords:
Carbapenem-resistant Klebsiella pneumoniae;
Allicin;
RNA sequencing;
Sugar transport system;
Bio-film;
Cluster analysis
- From:
Chinese Journal of Nosocomiology
2025;35(12):1765-1769
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the in vitro inhibitory mechanism of allicin against carbapenem-resistant Klebsiella pneumoniae(CRKP).METHODS The standard microbroth dilution method was employed to determine the minimum inhibitory concentration(MIC)of allicin against the experimental CRKP.Growth curve experiments were conducted to verify the relationship between allicin concentration and antibacterial action.RNA sequencing(RNA-seq)was performed on allicin-treated CRKP and a normal control group to explore the molecular mecha-nism of allicin inhibiting CRKP growth.RESULTS The MIC of allicin against CRKP was 140 mg/ml.A total of 1 775 differentially expressed genes were screened from the normal control group and the allicin-treated group,with 845 genes upregulated and 930 genes downregulated.The expression of bacterial phosphotransferase system(PTS)genes showed significant differences between the control group and the treated group(P＜0.05),and the expression levels of EIIA-EIID genes decreased(P＜0.05).CONCLUSION Based on the analysis of RNA-seq re-sults,it is found that allicin may inhibits the growth of CRKP by disrupting its sugar transport system,thereby affecting its biofilm formation.
