Efficacy of artificial tiger bone powder in the treatment of knee osteoarthritis with Wnt/β-catenin pathway
10.3969/j.issn.1673-9701.2025.16.015
- VernacularTitle:人工虎骨粉通过Wnt/β-catenin通路治疗膝骨关节炎的疗效
- Author:
Yichao WU
1
;
Shengting ZHANG
1
;
Ao GUO
1
;
Cong SUI
1
Author Information
1. 安徽医科大学第一附属医院骨科,安徽 合肥 230022
- Publication Type:Journal Article
- Keywords:
Knee osteoarthritis;
Artificial tiger bone powder;
Wnt/β-catenin pathway;
Molecular mechanisms;
Treatment
- From:
China Modern Doctor
2025;63(16):62-68,98
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the efficacy of artificial tiger bone powder in the treatment of knee osteoarthritis(KOA)through Wnt/β-catenin pathway.Methods Forty SD rats were divided into control group,KOA group,low-dose group and high-dose group.The KOA group,low-dose group and high-dose group were all modeled by anterior cruciate ligament transection.After modeling,high-dose and low-dose groups were treated with artificial tiger bone powder at a dose of 10g/kg and 5g/kg orally respectively.Cartilage tissue specimens of rats in each group were collected for hematoxylin-eosin staining and immunohistochemical staining,and Mankin score was conducted.The cartilage tissues of the knee joints of normal SD rats were taken for primary chondrocyte culture.After passage,they were divided into blank group(without any stimulation),interleukin-1β(IL-1β)group(incubated with 10ng/ml IL-1β for 1 day),and IL-1β+artificial tiger bone powder group(incubated with 10ng/ml IL-1β and 10μg/ml artificial tiger bone powder for 1 day).Detect the activities of related substances such as typeⅡ collagen(Col Ⅱ),matrix metalloproteinase-13(MMP-13),and β-catenin in each group.Results Compared with KOA group,after artificial tiger bone powder treatment,the degree of redness and swelling of the knee joints in rats was reduced,the range of joint motion was significantly improved,and the levels of inflammatory factors were decreased.Compared with the IL-1β group,the expression levels of Col Ⅱ protein and mRNA in IL-1β+artificial tiger bone powder group were significantly increased(P＜0.05),while the expression levels of MMP-13,β-catenin protein and mRNA decreased significantly(P＜0.05).After adding the Wnt agonist LiCL,the mRNA expression levels of Wnt1,β-catenin and MMP-13 increased,while the addition of the Wnt inhibitor WIF1 could reduce the mRNA expression levels of Wnt1,β-catenin and MMP-13.Conclusion Artificial tiger bone powder can inhibit the progression of KOA by regulating Wnt/β-catenin pathway.
