The role of immature neutrophils in controlling chronic Plasmodium chabaudi infection
10.13431/j.cnki.immunol.j.20240104
- VernacularTitle:幼稚中性粒细胞在疟原虫慢性感染中的作用研究
- Author:
Jiaqin FANG
1
;
Taiping LIU
1
;
Wenyue XU
1
Author Information
1. 404100 重庆,陆军军医大学基础医学院病原生物学教研室
- Publication Type:Journal Article
- Keywords:
Neutrophils;
Malaria;
Chronic infection;
Monocytes
- From:
Immunological Journal
2024;40(10):745-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the effect of immature neutrophils on chronic Plasmodium chabaudi(P.c)infection.Methods Single-cell sequencing combined with flow cytometry was used to detect dynamic changes in immature neutrophils and its S100A9 protein expression during P.c infection.Mice were intragastrically administered Paquinimod to deplete the immature neutrophils.Flow cytometry was used to assess its effect on the immune response against Plasmodium chabaudi.Single-cell sequencing and flow cytometry were conducted on splenocytes to examine changes of monocytes/macrophages.Results Clodronate liposomes(CLs)were administered and parasitemia and survival rates were monitored.The data showed that the frequency and number of immature neutrophils increased significantly during the chronic phase of P.c infection.Parasitemia was significantly elevated in the drug-treatment group,accompanied by a reduction in the frequency and number of immature neutrophils and monocytes.After CLs treatment,monocytes and macrophages decreased and parasitemia increased.Conclusion Taken together,a large number of immature neutrophils are newly generated during the chronic phase of Plasmodium chabaudi infection,and they may exert an anti-Plasmodium chabaudi protective role through an impact on monocytes.
