Shaoyao Gancao Decoction Combined with Electroacupuncture Against Poststroke Spasticity in Rats
- VernacularTitle:电针联合芍药甘草汤抑制卒中大鼠肢体痉挛
- Author:
Ying SHANG
1
;
Yaqi HUANG
;
Fei WANG
;
Hailong ZHAO
;
Shengfu ZHANG
;
Nenggui XU
;
Bin GUO
Author Information
- Publication Type:Journal Article
- Keywords: Electroacupuncture; Shaoyao Gancao decoction; Yanglingquan; Qu Chi; Stroke; Limb spasticity
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(4):982-991
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the effect of Shaoyao Gancao decoction combined with electroacupuncture on poststroke spasticity in rats.Methods Healthy male Sprague-Dawley(SD)rats were randomly divided into blank group,model group,electroacupuncture group,baclofen group,electroacupuncture combined with high dose of Chinese medicine group,electroacupuncture combined with middle dose of Chinese medicine group,electroacupuncture combined with low dose of Chinese medicine group,high dose of Chinese medicine group,middle dose of Chinese medicine group and low dose of Chinese medicine group,with 10 rats in each group.Open field test and integrated electromyography of quadriceps femoris were tested before modeling and on the 1st,3rd and 6th day after modeling.The samples were taken on day 6.The expression of inflammatory factors in M1 cortex were detected by liquid phase factor technique.The expression of brain-derived neurotrophic factor(BDNF),synaptophysin(SYP),postsynaptic density 95(PSD95),Nestin and β-catenin in M1 cortex was detected by ELISA and Western blot.Results Compared with model group and other intervention groups,the electroacupuncture combined with middle dose of Chinese medicine group make best total distance(P=0.021)and average speed(P=0.021)in open field test on 6th day.make better in integrated electromyography of quadriceps femoris test(P=0.006)on day 3,have higher Ⅱ type fiber ratio,promote IL-10(P=0.006),GMcsf(P=0.045)secretion.ELISA and Western blot also showed that this group behave better in the test of BDNF,SYP,PSD95,Nestin and β-catenin.Conclusion Electroacupuncture combined with Shaoyao-Gancao decoction groups were effective in the treatment of poststroke spasticity in rats,and its mechanism may be to improve the inflammatory environment of the injured site,promote the proliferation of neural stem cells and synaptic regeneration.
