miR-223,NLRP3 and Caspase-1 mRNA of thoracolumbar vertebral fracture patients with postoperative incision infections and their diagnostic values
10.11816/cn.ni.2025-241279
- VernacularTitle:胸腰椎骨折术后切口感染miR-223、NLRP3、Caspase-1 mRNA水平及其诊断价值
- Author:
Baoming ZHAN
1
;
Changpeng LIU
1
;
Yingguo QI
1
;
Shiqiang FANG
1
;
Xuesheng XIE
1
Author Information
1. 山东第一医科大学附属人民医院脊柱外科,山东济南 271100
- Publication Type:Journal Article
- Keywords:
Thoracolumbar vertebral fracture;
Incision infection;
Microribonucleic acid-223;
Nucleotide-binding ol-igomerized domain-like receptor 3;
Effect protein Caspase-1;
Serum amyloid A;
Monocyte chemoattractant pro-tein-1;
Toll-like receptor-2
- From:
Chinese Journal of Nosocomiology
2025;35(9):1347-1351
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the expression levels of microribonucleic acid-223(miR-223),nucleotide-bind-ing oligomerized domain-like receptor 3(NLRP3)and effect protein Caspase-1(Caspase-1)messenger ribonucleic acid(mRNA)in the thoracolumbar vertebral fracture patients with postoperative incision infections and analyze the diagnostic values.METHODS A total of 222 patients with thoracolumbar vertebral fracture who were treated in Jinan People's Hospital from Jan.2021 to Dec.2022 were enrolled in the study and were divided into the infection group with 25 cases and the no infection group with 197 cases according to the status of postoperative infections.The relative expression levels of peripheral blood miR-223,NLRP3 and Caspase-1 mRNA were detected by means of real-time fluorescent quantitative polymerase chain reaction(qPCR),the values of the three indexes in predic-tion of the postoperative incision infections were analyzed by means of receiver operating characteristic(ROC)curves,and Pearson correlation analysis was performed for the linear relationship between the miR-223,NLRP3 and Caspase-1 and the serum amyloid A(SAA)protein,monocyte chemoattractant protein-1(MCP-1)and Toll-like receptor-2(TLR-2).RESULTS There were significant differences in the levels of miR-223,NLRP3 and Caspase-1 mRNA between the infection group and the no infection group(P＜0.05);the levels of the above inde-xes of the infection group were 3.01±1.00,2.25±0.75 and 2.31±0.77,respectively.The area under the curve(AUC)of the joint detection of miR-223,NLRP3 and Caspase-1 was 0.935 in prediction of the postoperative inci-sion infections in the patients with thoracolumbar vertebral fracture,with the sensitivity 92.00％,the specificity 80.70％.There were significant differences in the levels of SAA,MCP-1 and TLR-2 between the infection group and the no infection group(P＜0.05);the levels of the above indexes of the infection group were(169.74±19.64)mg/L,(60.45±14.63)ng/L and(75.73±13.63)μg/L,respectively.The levels of miR-223,NLRP3 and Caspase-1 mRNA are positively correlated with SAA,MCP-1 and TLR-2(P＜0.05).CONCLUSIONS The chan-ges of miR-223,NLRP3 and Caspase-1 mRNA are associated with the postoperative incision infections in the pa-tients with thoracolumbar vertebral fracture.The joint detection of miR-223,NLRP3 and Caspase-1 mRNA may facilitate the diagnosis of postoperative incision infections,and the three indexes are positively correlated with SAA,MCP-1 and TLR-2.
