Phenotypes and genotypes of 85 strains of carbapenem-resistant Enterobacteriaceae
10.11816/cn.ni.2025-246686
- VernacularTitle:85株耐碳青霉烯类肠杆菌科细菌表型及基因型分布
- Author:
Meina WANG
1
;
Qinglan MENG
;
Junrui WANG
;
Wenqi ZHENG
;
Quan FU
;
Yan GU
Author Information
1. 内蒙古医科大学附属医院呼吸与危重症医学科,内蒙古呼和浩特 010050
- Publication Type:Journal Article
- Keywords:
Carbapenem-resistant Enterobacteriaceae;
Phenotype;
Genotype;
Sequence type;
Prevalence strain
- From:
Chinese Journal of Nosocomiology
2025;35(9):1299-1304
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To detect the phenotypes and genotypes of carbapenem-resistant Enterobacteriaceae(CRE)strains and understand the drug resistance phenotypes and molecular characteristics of the CRE strains.METHODS Totally 85 strains of CRE were isolated and restored in microbiology lab of Affiliated Hospital of Inner Mongolia Medical University from Jan.2019 to Aug.2023.The phenotypes of carbapenemases were detected by means of carbapenemase inhibitor enhancement test,the genotypes of carbapenemases were detected by polymerase chain reaction(PCR),and the sequence type(ST)was further analyzed by multilocus sequence typing(MLST).RESULTS Among the 85 strains of CRE that were detected for phenotypes,54 strains carried serinase(type A carbapenemase),23 strains carried metalloenzyme(type B carbapenemase),5 strains produced both type A and type B carbapenemase,and 3 strains produced neither the type A and/or type B carbapenemase.Totally 5 common genotypes of carbapenemases were amplified for 73 strains of CRE,the detection rate of blaKPC was highest,fol-lowed by blaNDM;the drug resistance genes blaIMP,blaVIM and blaOXA-48 were not detected.The 51 strains of car-bapenem-resistant Klebsiella pneumoniae(CRKP)were classified into 7 types of ST in total,which were as fol-lows:ST1,ST11,ST15,ST23,ST34,ST690,ST4862.CONCLUSIONS The isolation rate of CRKP is highest among the CRE strains,and ST11 is the most prevalent strain.Serinase is the most common drug resistance phe-notype,and KPC type carbapenemase is the predominant drug resistance genotype.
