GBP3 negatively regulates HTLV-1 replication in a GTPase-dependent manner
10.3969/j.issn.1000-484X.2025.03.004
- VernacularTitle:GBP3依赖其GTP酶活性负向调控HTLV-1病毒复制
- Author:
Yanzi LIU
1
;
Xin ZHAO
;
Xiao QIN
;
Yulu HUANG
;
Xi YANG
;
Qingqing FAN
;
Bo YANG
;
Jie WANG
Author Information
1. 新乡医学院第三附属医院检验科,新乡医学院医学技术学院,新乡 453003
- Publication Type:Journal Article
- Keywords:
GBP3;
HTLV-1;
Antivirus;
GTPase
- From:
Chinese Journal of Immunology
2025;41(3):535-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of GBP3 on replication of adult T-lymphocytic leukemia virus type 1(HTLV-1).Methods:Expression of GBP3 in HTLV-1-infected HeLa cell and THP1 cell was detected by Western blot.The knock-down efficiency of siRNAs targeting GBP3 in HeLa and THP1 cells was evaluated by Western blot.Effects of GBP3 overexpression or knockdown on expression of HTLV-1 proviral transcripts Tax,px,HBZ,Gag,ENV,5'UTR,and viral proteins Tax,p19 were investigated by RT-qPCR and Western blot.GTPase-defective mutant of GBP3,GBP3K51A was constructed to explore whether the effects of GBP3 on HTLV-1 infection were dependent on its GTPase activity.Results:GBP3 expression was upregulated in HTLV-1 infected HeLa and THP1 cells.GBP3 overexpression decreased expression of HTLV-1 proviral transcripts and viral proteins,whereas the knockdown of GBP3 has the opposite effects.Overexpression of GBP3K51A increased expression of HTLV-1 proviral transcripts and viral proteins.Conclusion:HTLV-1 virus infection can induce expression of GBP3;overexpression of GBP3 inhibits virus replication and may depend on GTPase.
