Effect of knockout ST2 expression on intestinal microflora in mice with ConA-induced autoimmune hepatitis
10.3969/j.issn.1000-484X.2025.03.003
- VernacularTitle:敲除ST2表达对ConA诱导的自身免疫性肝炎小鼠肠道菌群的影响
- Author:
Renquan JIANG
1
;
Jifeng TANG
1
;
Yujue HE
1
;
Jinfang XIA
1
;
Jinpiao LIN
1
;
Qishui OU
1
Author Information
1. 福建医科大学附属第一医院检验科,福建医科大学附属第一医院滨海院区国家区域医疗中心检验科,福建省检验医学重点实验室,福建医科大学基因诊断研究中心,福建省临床免疫学检验临床医学研究中心,福州 350005
- Publication Type:Journal Article
- Keywords:
ST2 knockout mice;
ConA;
Intestinal flora;
Autoimmune hepatitis;
Diagnostic value
- From:
Chinese Journal of Immunology
2025;41(3):529-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of growth stimulation expressed gene 2(ST2)on intestinal microflora of mice with ConA-induced autoimmune hepatitis(AIH)by knockout.Methods:ST2 gene knockout mice were constructed by gene knockout technique.On this basis,AIH mouse model was established by ConA induction.The relative expression of cytokines was detected by real-time fluorescence quantitative PCR(RT-PCR).Intestinal microorganisms were sequenced using the 16S rRNA high-through put sequencing method,and the sequencing results were analyzed using Microbial Ecology related software and database.Results:Com-pared with the control group,ALT and AST were decreased,the damage of liver was mild,and the relative expression of IL-6 was also decreased in the liver of mice with ConA-induced AIH after ST2 gene knockout.There was no significant difference in the ACE and CHAO indices of the abundance of reactive flora.There was no significant difference between Shannon index and Simpson index re-flecting bacterial diversity.Psychrobacter,unclassified_Clostridia,Halothiobacillus,Clostridium_XlVa and Haemophilus genus content increased;Romboutsia,Rikenella,Parabacteroides and unclassified_Clostridiales bacterial content reduce.The areas under the receiv-er operator characteristic(ROC)curves were 0.88,0.89,0.88,0.80,0.90,0.90,0.84,0.91 and 0.84,respectively.Conclusion:Knockout of ST2 gene expression in mice with AIH can reduce liver injury and inflammation,and does not affect the distribution abun-dance and diversity of intestinal flora.However,there are differences among bacteria genera,and it has good diagnostic value.
