Riboflavin reduces the range of ischemic stroke infarction by inhibiting the neuronal apoptosis in mice
10.16557/j.cnki.1000-7547.2025.01.004
- VernacularTitle:核黄素通过抑制缺血性脑卒中小鼠神经元凋亡缩小梗死范围
- Author:
Wei YANG
1
;
Juan PANG
;
Yuhang XIA
;
Jun LI
;
Han YANG
;
Fenqing SHANG
;
Junru YIN
Author Information
1. 西安市胸科医院转化医学中心,西安 710100
- Publication Type:Journal Article
- Keywords:
riboflavin;
cerebral ischemic stroke;
middle cerebral artery occlusion;
neuronal apoptosis;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(1):25-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of riboflavin on cerebral infarction volume and the possible mecha-nism of apoptotic factors with cerebral ischemic injury in mice.Methods:Eighteen C57BL/6J male mice were divided into the sham group,middle cerebral artery occlusion(MCAO)group and riboflavin intervention group(MCAO+RF)randomly.TTC staining was used to observe the infarction of the cerebral tissues;Quantitative real-time PCR(RT-qPCR)was used to detect the mRNA expression of tumor protein p53(p53),cytochrome C(CytC),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X(Bax),cysteinyl aspartate specific proteinase-3(caspase-3),poly ADP-ribose poly-merase(PARP),cysteinyl aspartate specific proteinase-6(caspase-6)and apoptosis inducing factor(AIF)in different groups,to study the possible mechanism of riboflavin inhibiting neuronal apoptosis.The proteins expression of acetyl-p53(AC-p53),caspase-3 and PARP were analyzed by Western blot.Results:Compared with the MCAO group,the cerebral infarct volume of the MCAO+RF group was obviously reduced(P＜0.01);The relative expression of p53,CytC,caspase-3,PARP,caspase-6 and AIF were significantly lower in the MCAO+RF group(P＜0.05).Addition-ally,significant differences were observed in the proteins expression of AC-p53,caspase-3 and PARP between the MCAO group and MCAO+RF group.Conclusion:Riboflavin has a protective effect against cerebral ischemic injury,which is possibly realized by inhibiting neuronal apoptosis through multiple pathways.
