Neurons in the paraventricular nucleus of the thalamus in mice are involved in memory impairment due to neuropathic pain
10.16557/j.cnki.1000-7547.2025.01.003
- VernacularTitle:小鼠丘脑室旁核神经元参与神经病理性痛导致的记忆障碍
- Author:
Changlei ZHU
1
;
Jingjing TIE
;
Feifei WU
;
Yanling YANG
;
Yayun WANG
Author Information
1. 空军军医大学基础医学院神经系统疾病线粒体可塑性实验室,国家级基础医学实验教学示范中心,西安 710032
- Publication Type:Journal Article
- Keywords:
peripheral nerve injury(PNI);
neuropathic pain(NP);
memory impairment;
c-FOS;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(1):18-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of peripheral nerve injury(PNI)on neuropathic pain(NP)and memo-ry function in mice,as well as the activation of neurons in the paraventricular nucleus(PVT)of the thalamus,so as to provide a basis for studying the relationship between NP and memory impairment.Methods:Twenty one 8-week-old male C57 BL/6J mice were randomly divided into sham group and experimental group,and the routine spared nerve inju-ry(SNI)was constructed in the mice of experimental group.The pain behavior and memory impairment of mice after SNI were evaluated with hot plate and eight-arm maze behavioral tests.Pearson correlation analysis was performed to an-alyze the correlation between pain behavior and memory impairment.The c-FOS expression in PVT was detected with immuno-staining.Results:Compared with the sham group,the heat pain threshold of mice in the experimental group was significantly reduced(P＜0.001).The results of the eight-arm maze test showed that the total rest time was signifi-cantly increased(P＜0.001),and the working memory error was increased from 1 to 4 days after SNI(P＜0.01).Correlation analysis indicated that early working memory errors were negatively correlated with heat pain threshold after SNI(P＜0.001).The immunofluorescence revealed that the number of c-FOS positive cells in PVT increased signifi-cantly(P＜0.001).Conclusion:SNI can cause abnormal pain behavior and memory impairment in mice,and cause neuronal activation in PVT.This study provides a basis for neurons in PVT to participate in the regulation of memory impairment in the context of NP.
