Chemical constituents from Gymnema tingens and their in vitro hypoglycemic activity
10.3969/j.issn.1001-1528.2025.06.018
- VernacularTitle:大叶匙羹藤化学成分及其体外降血糖活性研究
- Author:
Mei-yu LIU
1
;
Xin ZHAN
;
Guang-feng LIAO
;
Jin-yan ZHANG
;
Xin-zhou YANG
;
Ru-mei LU
Author Information
1. 广西中医药大学药学院,广西南宁 530001
- Publication Type:Journal Article
- Keywords:
Gymnema tingens Spreng.;
chemical constituents;
isolation and identification;
hypoglycemic activity;
7-desoxyneocynapanogenin A;
C21 steroidal glycosides
- From:
Chinese Traditional Patent Medicine
2025;47(6):1892-1900
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Gymnema tingens Spreng.and their in vitro hypoglycemic activity.METHODS The 70％ethanol extract was isolated and purified by macroporous resin,silica gel,sephadex LH-20,and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical propeties and spectral data.The in vitro hypoglycemic activity was evaluated by glucose uptake test in L6 cells.RESULTS Seventeen compounds were isolated and identified as 7-desoxyneocynapanogenin A(1),glaucogenin(2),cynatratoside A(3),atratcynoside F(4),(+)-lyoniresinol(5),(+)-lyoniresinol 3-O-α-D-rhamnopyranoside-(1→6)-β-D-glucopyranoside(6),fernandoside(7),3,4-dimethoxy-phenyl-1-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(8),khaephuoside A(9),khaephuoside B(10),3,4,5-trimethoxy-phenyl-O-β-D-glucopyranoside(11),liquiritigenin(12),7,3'-dihydroxy-flavanone-4'-O-β-D-glucopyranoside(13),pinoresinol(14),syringaldehyde(15),(+)-1-hydroxy-pinoresinol-1-β-D-glucopyranoside(16),β-amyrin(17).Compounds 2-5、7、9、10、12、17 could promote the glucose uptake in L6 cells.CONCLUSION Compound 1 is a new compound,and 2-9、11-13、15-17 are isolated from this plant for the first time.Compounds 2-5、7、9、10、12、17 have good hypoglycemic activity.
