Comprehensively Evaluate the Quality of Hypericum Sampsonii by QAMS Multi-component Quantification and PCA,OPLS-DA,EW-TOPSIS Analysis
10.16466/j.issn1005-5509.2024.11.005
- VernacularTitle:QAMS多成分定量及PCA、OPLS-DA、EW-TOPSIS分析综合评价元宝草药材质量
- Author:
Jianfang ZHANG
1
;
Zongliang CHEN
;
Yingying DONG
Author Information
1. 绍兴市第七人民医院 浙江,绍兴 312000
- Publication Type:Journal Article
- Keywords:
Hypericum sampsonii;
HPLC-QAMS;
chemical mode identification;
PCA;
OPLS-DA;
EW-TOPSIS;
quality evaluation
- From:
Journal of Zhejiang Chinese Medical University
2024;48(11):1363-1376
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To establish a method for simultaneous determination of 12 components of Hypericum sampsonii from different producing areas,and evaluate its quality by chemometrics and entropy weight-technique for order preference by similarity to an ideal solution(EW-TOPSIS).[Methods]The analysis was performed on a Agilent 5 TC-C18 column with mobile phase consisting of 0.5％ phosphoric acid-acetonitrile solution(gradient elution)at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm and 210 nm.Relative correction factor(RCF) for rutoside,hyperoside,quercetin,kaempferol,3,8'-biapigenin,euxanthone,1,3-dihydroxy-5-methoxy-xanthone,norathyriol,emodin,physcion,hyperforin and mangiferin was established with mangiferin as internal reference,and the contents were calculated,and 12 components were determined by external standard method(ESM) method.Chemical mode identification and EW-TOPSIS method were used to establish the quality evaluation model of Hypericum sampsonii,and the overall quality was evaluated comprehensively.[Results]The methodological examination results of the external standard method all met the requirements of Chinese Pharmacopoeia.There was no significant difference in the content results between the QAMS method and the ESM.The principal component analysis(PCA) results showed that 18 batches of samples were clustered into 3 categories;the orthogonal partial least squares-discriminant analysis(OPLS-DA) analysis results showed that 18 batches of samples were significantly classified,and 5 differential biomarkers that caused differences in the quality of Hypericum sampsonii from different regions were screened out;EW-TOPSIS analysis results showed that the overall quality of Hypericum sampsonii from Sichuan,Guizhou and Guangxi provinces were better.[Conclusion]The established HPLC-QAMS method for simultaneous determination of 12 components in Hypericum sampsonii are convenient and accurate.Chemical mode identification and EW-TOPSIS method are objective and comprehensive,which can be used for the overall quality evaluation of Hypericum sampsonii.
