Effects of amanita caojizong on apoptosis of mouse myocardial cells and expression of related factors Bcl-2 and Bax
10.13618/j.issn.1001-5728.2025.02.007
- VernacularTitle:隐花青鹅膏对小鼠心肌细胞凋亡及其相关因子Bcl-2、Bax表达的影响
- Author:
Baining QIU
1
;
Yuebing WANG
;
Sijie WEI
;
Wu LONG
;
Rui WANG
;
Lin MA
;
Yanmei XI
;
Xue TANG
;
Puping LEI
Author Information
1. 昆明医科大学法医学院,云南 昆明 650500
- Publication Type:Journal Article
- Keywords:
Forensic toxicology;
Mitochondrial apoptosis pathway;
Mouse myocardial cells;
Amanita caojizong;
Bax/Bcl-2
- From:
Chinese Journal of Forensic Medicine
2025;40(2):168-171,180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Amanita caojizong on cardiomyocyte apoptosis and the expression of apoptosis-related factors Bcl-2 and Bax,thereby providing experimental evidence for the prevention and treatment of Amanita caojizong poisoning.Methods Mouse cardiomyocytes(HL-1 cells)cultured in vitro were divided into an experimental group(treated with Amanita caojizong extract)and a control group(treated with PBS).After treatment with Amanita caojizong extract,apoptosis of HL-1 cells was observed using TUNEL staining,and the protein expression levels of Bax,Bcl-2,Caspase-3,and Cleaved Caspase-3 in HL-1 cardiomyocytes were detected by Western blot.Results Compared with the control group,the TUNEL staining showed significantly increased apoptotic fluorescence intensity in the Amanita caojizong extract-treated group.The protein expressions of Bax,Caspase-3,and Cleaved Caspase-3 in HL-1 cells in the Amanita caojizong-treated group were upregulated,while the expression of Bcl-2 was downregulated.Conclusion Amanita caojizong can promote apoptosis of mouse cardiomyocytes,and its mechanism may be associated with the Bcl-2/Bax pathway.
