Experimental study on the impact of nucleic acid purification and vacuum concentration on DNA recovery rate
10.13618/j.issn.1001-5728.2025.02.020
- VernacularTitle:核酸纯化仪结合真空浓缩仪对DNA回收率影响的研究
- Author:
Huajie BA
1
;
Hao NIE
;
Xingchen ZHANG
;
Aihua ZHU
;
Ming JIN
Author Information
1. 常州市公安局物证鉴定所,江苏 常州 213003
- Publication Type:Journal Article
- Keywords:
Forensic biology;
DNA extraction;
DNA concentration;
Vacuum concentrator;
Nucleic acid purifier
- From:
Chinese Journal of Forensic Medicine
2025;40(2):241-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of a nucleic acid purifier combined with a vacuum concentrator on DNA recovery rate.Methods Using 39 tubes of 007 standard samples,two 30 μL samples were taken from each tube.Samples were purified using the PrepFiler ExpressTM BTA Purification Kit on the AutoMate ExpressTM purifier,with elution volumes of 30 μL and 200 μL.Samples with 200 μL eluent were concentrated to 30 μL using a trace DNA sample vacuum concentrator.DNA solution concentrations were measured,and recovery rates were calculated and compared.Results The measured DNA concentration of the 007 standard samples was 0.08～0.15(0.11±0.02)ng/μL,showing a significant difference from the kit-labeled concentration of 0.1 ng/μL(t=3.88,P<0.01).The DNA solution concentration in the purified and concentrated group(200 μL eluent concentrated to 30 μL)[0.05～0.13(0.08±0.02)ng/μL]was significantly higher than the purified group(30 μL eluent)[0.02～0.08(0.05±0.01)ng/μL](t=21.20,P<0.01).The DNA recovery rate of the purified and concentrated group[54.55%～92.86%(75.41±10.12)%]was substantially higher than the purified group[25.00%～54.55%(39.79±7.13)%](t=19.79,P<0.01).Conclusion Utilizing a large-volume elution system in nucleic acid purification can significantly enhance DNA recovery rate.Combining this method with a vacuum concentrator effectively increases DNA solution concentration,thereby improving DNA testing success rates.
