UPLC-MS/MS detection of four synthetic cannabinoids and their metabolites in urban wastewater
10.13618/j.issn.1001-5728.2025.02.015
- VernacularTitle:UPLC-MS/MS法检测污水中4种合成大麻素及其代谢产物
- Author:
Xinyi LI
1
;
Taoren WANG
;
Deyun NIU
;
Yu XU
;
Bin LI
;
Jiaxue SUN
;
Dan XUE
;
Hong LI
Author Information
1. 昆明医科大学法医学院,云南 昆明 650500
- Publication Type:Journal Article
- Keywords:
Forensic toxicology analysis;
Detection method;
Solid-phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry;
Urban wastewater;
Synthetic cannabinoids
- From:
Chinese Journal of Forensic Medicine
2025;40(2):213-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of offline solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry for detecting four synthetic cannabinoids(5F-ADB,5F-MDMB-PICA,4F-MDMB-BUTINACA,and MDMB-4en-PINACA)and their marker metabolites in urban wastewater.Methods Samples were pre-treated using an Oasis PRiME HLB column(60 mg,3CC)and analyzed by ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry.The mobile phases consisted of 0.02%formic acid-2 mmol/L ammonium formate aqueous solution and acetonitrile solution,with a WATERS ACQUITY UPLCHSS T3 column.Analysis was conducted in positive ionization mode of the ESI ion source,combining multiple reaction monitoring(MRM),information-dependent acquisition(IDA),and enhanced product ion(EPI)scanning modes for qualitative and quantitative analysis of the eight target compounds.Results Good linearity was observed for 4F-MDMB-BUTINACA-M,5F-ADB,5F-ADB-M,and 5F-MDMB-PICA in the concentration range of 1～250 ng/L;for MDMB-4en-PINACA in the range of 5～250 ng/L;and for 4F-MDMB-BUTINACA,5F-MDMB-PICA-M,and 4F-MDMB-BUTINACA-M in the range of 1～500 ng/L.The recovery rates of samples spiked with different concentrations of the eight target compounds ranged from 87.68%to 104.68%,with matrix effects between 88.61%and 112.78%.Both intra-day and inter-day precision were less than 5%.Conclusion The established method demonstrates good accuracy and reproducibility,making it suitable for qualitative and quantitative detection of four synthetic cannabinoids and their metabolites in urban wastewater.
