Microbial characteristics analysis of the lungs in children with community-acquired pneumonia of different severity levels
10.3969/j.issn.1673-9701.2024.36.005
- VernacularTitle:不同危重程度社区获得性肺炎患儿肺部微生物特征分析
- Author:
Yong WU
1
;
Xiuxia PAN
1
;
Hua QIN
1
;
Yunjun LIU
1
;
Yan ZHU
1
;
Sijia WANG
1
;
Yonghua LIANG
1
;
Rong ZENG
1
;
Qian WU
1
Author Information
1. 荆门市人民医院儿科,湖北荆门 448000
- Publication Type:Journal Article
- Keywords:
Bronchoalveolar lavage fluid;
Metagenomic sequencing;
Community acquired pneumonia;
Pulmonary microbiome
- From:
China Modern Doctor
2024;62(36):22-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study microbial characteristics of pulmonary in children with community-acquired pneumonia(CAP)of different severity,in order to provide a basis for accurate diagnosis and antibiotic treatment of pneumonia children,and provide new strategies and perspectives for the diagnosis and treatment of pulmonary microbiota in pneumonia children.Methods Bronchoalveolar lavage fluid(BALF)from 64 children with CAP of different severity hospitalized in Department of Pediatrics,Jingmen People's Hospital were collected from January to December 2023,the children were divided into severe pneumonia group(n=34)and common pneumonia group(n=30).Microbiome information of the lungs of children with CAP of different severity were obtained through metagenomic sequencing of BALF,microbial structure diversity analysis,species classification analysis,and differential analysis on the microbial bioinformatics data of two groups of samples obtained were performed.Results Alpha diversity analysis showed that there were statistically significant differences(P<0.05)in the Chao1 index,ACE index,Shannon index,and Simpson index between two groups.The principal coordinate analysis(PCoA)of Beta diversity showed a statistically significant difference in the composition of microbial communities between two groups(F=4.221,P=0.005).Through species classification analysis,it was found that at the genus level,mycoplasma was the main genus in the BALF samples of severe pneumonia group,followed by Streptococcus and Haemophilus,Streptococcus was the main genus in the BALF samples of common pneumonia group,followed by Mycoplasma and Haemophilus.Children of two groups showed statistically significant differences in microbial abundance among the top 20 species at the genus level(P<0.05),including Mycoplasma,Streptococcus,Rhodococcus,Neisseria,Prevotella,Corynebacterium,and Pseudomonas.Species diversity analysis showed that at the genus level,there were 47 species with differences(P<0.05).Conclusion There are differences in the abundance,diversity,structure,and composition of pulmonary microbiota in children with CAP of different severity.The dominant microbiota varies among children with CAP of different severity.This study enriches the pulmonary microbiome data of children with CAP.
