Determination of etomidate content in blood by GPC-GCMS method
- VernacularTitle:血液中依托咪酯含量的GPC-GCMS法测定
- Author:
Shanchuan HE
1
;
Ming JIANG
1
;
Min HUANG
1
;
Wang SHI
1
;
Libin LIU
1
;
Bin WANG
1
;
Lei WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Blood; Etomidate; GPC-GCMS; External standard curve method; Determination of content
- From: Chinese Journal of Forensic Medicine 2025;40(2):199-202,212
- CountryChina
- Language:Chinese
- Abstract: Objective To establish an online gel permeation chromatography-gas chromatography/mass spectrometry(GPC-GCMS)method for the determination of etomidate in blood.Methods Blood samples were extracted with acetonitrile,dehydrated with anhydrous sodium sulfate,purified with an online GPC system,and then analyzed by GCMS using large volume injection.The external standard curve method was used for quantification.Results The detection limit of etomidate was 0.002 μg/mL,with good linearity in the concentration range of 0.01～5 μg/mL and a correlation coefficient of 0.9992.The extraction recovery rates ranged between 90%and 120%.In an actual case,the content of etomidate detected in the blood sample was 0.024 μg/mL.Conclusion This method is fast,simple,accurate,and stable,providing a reliable basis for the determination of etomidate content in blood.
