Simultaneous content determination of fourteen active constituents in Xiao'er Feike Granules by UPLC-MS/MS
10.3969/j.issn.1001-1528.2025.03.002
- VernacularTitle:UPLC-MS/MS法同时测定小儿肺咳颗粒中14种活性成分的含量
- Author:
Feng ZHANG
1
;
Yan LIANG
;
Meng-meng ZHANG
;
Ying CAO
Author Information
1. 开封市食品药品质量安全中心,河南开封 475000
- Publication Type:Journal Article
- Keywords:
Xiao'er Feike Granules;
active constituents;
content determination;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2025;47(3):716-721
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of betaine,calycosin-7-glucoside,rscogenin,cinnamaldehyd,atragaloside Ⅳ,6-gingerol,chrysophanic acid,naringin,hesperidin,ginsenoside Rg1,ginsenoside Rb1,glycyrrhizic acid,rhein and emodin in Xiao'er Feike Granules.METHODS The analysis was performed on a 35 ℃ thermostatic Phenomenex Kinetex C18 column(2.1 mm× 100 mm,2.6 μm),with the mobile phase comprising of 0.1％formic acid-acetonitrile flowing at 0.35 mL/min,and electron spray ionization source was adopted in positive and negative ion scanning with dynamic multiple reaction monitoring mode.RESULTS Fourteen active constituents showed good linear relationships within their own ranges(r＞0.996 0),whose average recoveries were 96.0％-101.6％with the RSDs of 2.4％-4.3％.CONCLUSION This stable,accurate and sensitive method can be used for the quality control of Xiao'er Feike Granules.
