Chemical constituents from the water fraction of rhizoma of Smilax trinervula and their biological activities
10.3969/j.issn.1001-1528.2025.03.016
- VernacularTitle:三脉菝葜根茎水部位化学成分及其生物活性研究
- Author:
Yong-hong LIANG
1
;
Jia-cheng WANG
;
Hui-lian HUANG
;
Hui-ying YAO
;
Yu LU
;
Cheng-qi WANG
;
Hai-ying ZHONG
;
Ying-cai YU
;
Hai-yan ZHANG
Author Information
1. 江西中医药大学现代中药制剂教育部重点实验室,江西南昌 330004
- Publication Type:Journal Article
- Keywords:
Smilax trinervula Miq.;
rhizoma;
water fraction;
chemical constituents;
isolation and identification;
anti-tumor activities;
α-glucosidase;
polyhydroxylated alkaloids
- From:
Chinese Traditional Patent Medicine
2025;47(3):807-812
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the water fraction of rhizoma of Smilax trinervula Miq.and their biological activities.METHODS Polyamide,silica gel,Sephadex LH-20,ODS and semi-preparative HPLC were used for isolation and purification,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activities were determined by MTT mothod,and the inhibitory activities on α-glucosidase were determined by PNPG method.RESULTS Eleven compounds were isolated and identified as tyrosine(1),uridine(2),2-(2',3',4'-trihydroxybutyl)-6-(2",3",4"-trihydroxybutyl)-pyrazine(3),2-(1',2',3',4'-tetrahydroxybutyl)-6-(2",3",4"-trihydroxybutyl)-pyrazine(4),2-(1',2',3',4'-tetrahydroxybutyl)-5-(2",3",4"-trihydroxybutyl)-pyrazine(5),uracil(6),2-(1',2',3',4'-tetrahydroxybutyl)-5-(1",2",3",4"-tetrahydroxybutyl)-pyrazine(7),dioscin(8),shikimic acid(9),pyrazine(10),3,4-dihydroxyphenyethyl alcohol 8-O-β-D-glycopyranoside(11).The IC50 values of compounds 8 to human breast cancer cell MCF-7 was(2.36±0.26)μg/mL,and the IC50 values of compounds 3-5 and 7 to α-glucosidase were(1.54±0.15)-(10.53±0.38)μg/mL.CONCLUSION Compounds 1-7,10 are isolated from Smilax genus for the first time,and compound 9,11 are first isolated from this plant.Compound 8 has anti-tumor activity,and compounds 3-5,7 have α-glucosidase inhibitory activities.
